Results: Representative Intratumoral PD-1+Tim-3+CD8+ T cell Quantification and Analysis
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Conclusion
Trascrizione
The overall goal of this multiplexed immunofluorescence analysis is to automatically count cells based on their phenotype. This method allows multifluorescence and automatic cell counting to analyze the phenotype and function of tumor immune cells
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Studying tumor microenvironment may identify prognostic or predictive biomarkers of clinical response to immunotherapy. Presented here, is an innovative method based on in situ fluorescence multispectral imaging to analyze and count automatically various subpopulations of CD8+ T cells. This reproducible and reliable technique is suitable for large cohort analyses.