Multiplexed Immunofluorescence Analysis and Quantification of Intratumoral PD-1+ Tim-3+ CD8+ T CellsClémence Granier 1, Emeline Vinatier 2,3,4, Elia Colin 2,3, Marion Mandavit 2,3, Charles Dariane 2,3,5, Virginie Verkarre 6, Lucie Biard 7, Rami El Zein 2, Corinne Lesaffre 2, Isabelle Galy-Fauroux 2, Hélène Roussel 2,3,6, Eléonore De Guillebon 8, Charlotte Blanc 2,3, Antonin Saldmann 4, Cécile Badoual 2,6, Alain Gey *4, Éric Tartour *2,4
1PARCC-INSERM U970, Université Paris Descartes, 2INSERM U970, Université Paris Descartes, 3Equipe Labellisée Ligue Contre le Cancer, 4Department of Immunology, Hopital Européen Georges Pompidou, 5Department of Medical Urology, Hopital Européen Georges Pompidou, 6Department of Pathology, Hopital Européen Georges Pompidou, 7Université Paris Diderot Paris 7, 8Department of medical oncology, Hopital Européen Georges Pompidou
Studying tumor microenvironment may identify prognostic or predictive biomarkers of clinical response to immunotherapy. Presented here, is an innovative method based on in situ fluorescence multispectral imaging to analyze and count automatically various subpopulations of CD8+ T cells. This reproducible and reliable technique is suitable for large cohort analyses.
