Sachdev S. Sidhu

The Erbin PDZ domain binds with high affinity and specificity to the carboxyl termini of delta-catenin and ARVCF.

Rapid identification of small binding motifs with high-throughput phage display: discovery of peptidic antagonists of IGF-1 function.

Comprehensive functional maps of the antigen-binding site of an anti-ErbB2 antibody obtained with shotgun scanning mutagenesis.

A minimized M13 coat protein defines the requirements for assembly into the bacteriophage particle.

Origins of PDZ domain ligand specificity. Structure determination and mutagenesis of the Erbin PDZ domain.

Exploring protein-protein interactions with phage display.

Functional genomics of intracellular peptide recognition domains with combinatorial biology methods.

The functional binding epitope of a high affinity variant of human growth hormone mapped by shotgun alanine-scanning mutagenesis: insights into the mechanisms responsible for improved affinity.

Contributions of CDR3 to V H H domain stability and the design of monobody scaffolds for naive antibody libraries.

Comprehensive mutagenesis of the C-terminal domain of the M13 gene-3 minor coat protein: the requirements for assembly into the bacteriophage particle.

Definition of the consensus motif recognized by gamma-adaptin ear domains.

Bivalent antibody phage display mimics natural immunoglobulin.

Phage-displayed antibody libraries of synthetic heavy chain complementarity determining regions.

Comprehensive mutational analysis of the M13 major coat protein: improved scaffolds for C-terminal phage display.

High-affinity human antibodies from phage-displayed synthetic Fab libraries with a single framework scaffold.

Synthetic antibodies from a four-amino-acid code: a dominant role for tyrosine in antigen recognition.

Intramolecular cooperativity in a protein binding site assessed by combinatorial shotgun scanning mutagenesis.

A structure-based database of antibody variable domain diversity.

Molecular recognition by a binary code.

Alternative views of functional protein binding epitopes obtained by combinatorial shotgun scanning mutagenesis.

Tyrosine plays a dominant functional role in the paratope of a synthetic antibody derived from a four amino acid code.

Convergent and divergent ligand specificity among PDZ domains of the LAP and zonula occludens (ZO) families.

Comparative structural analysis of the Erbin PDZ domain and the first PDZ domain of ZO-1. Insights into determinants of PDZ domain specificity.

Comprehensive and quantitative mapping of energy landscapes for protein-protein interactions by rapid combinatorial scanning.

Activation of the proapoptotic death receptor DR5 by oligomeric peptide and antibody agonists.

M13 bacteriophage coat proteins engineered for improved phage display.

Synthetic therapeutic antibodies.

Full-length antibodies on display.

Exploring and designing protein function with restricted diversity.

Identifying specificity profiles for peptide recognition modules from phage-displayed peptide libraries.

Structural and functional analysis of the ligand specificity of the HtrA2/Omi PDZ domain.

High-throughput generation of synthetic antibodies from highly functional minimalist phage-displayed libraries.

Phage display for engineering and analyzing protein interaction interfaces.

The type III effector EspF coordinates membrane trafficking by the spatiotemporal activation of two eukaryotic signaling pathways.

Structural and functional analysis of the PDZ domains of human HtrA1 and HtrA3.

Comprehensive analysis of the factors contributing to the stability and solubility of autonomous human VH domains.

Synthetic antibodies for specific recognition and crystallization of structured RNA.

Regulation of a late phase of T cell polarity and effector functions by Crtam.

The intrinsic contributions of tyrosine, serine, glycine and arginine to the affinity and specificity of antibodies.

Submitting antibodies to binding arbitration.

A dominant conformational role for amino acid diversity in minimalist protein-protein interfaces.

Ubiquitin chain editing revealed by polyubiquitin linkage-specific antibodies.

A specificity map for the PDZ domain family.

Two-state selection of conformation-specific antibodies.

Inhibition of Wnt signaling by Dishevelled PDZ peptides.

The importance of being tyrosine: lessons in molecular recognition from minimalist synthetic binding proteins.

Crystal structure of full-length KcsA in its closed conformation.

Rapid evolution of functional complexity in a domain family.

Improving therapeutic efficacy of a complement receptor by structure-based affinity maturation.

Bayesian modeling of the yeast SH3 domain interactome predicts spatiotemporal dynamics of endocytosis proteins.

The functional capacity of the natural amino acids for molecular recognition.

MOTIPS: automated motif analysis for predicting targets of modular protein domains.

Structure of the complex between HER2 and an antibody paratope formed by side chains from tryptophan and serine.

Coevolution of PDZ domain-ligand interactions analyzed by high-throughput phage display and deep sequencing.

Functional complexes between YAP2 and ZO-2 are PDZ domain-dependent, and regulate YAP2 nuclear localization and signalling.

A portable RNA sequence whose recognition by a synthetic antibody facilitates structural determination.

Evolving specificity from variability for protein interaction domains.

Development of reagents and assays for the detection of pathogenic Burkholderia species.

The multiple-specificity landscape of modular peptide recognition domains.

Phage display of tissue inhibitor of metalloproteinases-2 (TIMP-2): identification of selective inhibitors of collagenase-1 (metalloproteinase 1 (MMP-1)).

Identification of specificity determining residues in peptide recognition domains using an information theoretic approach applied to large-scale binding maps.

Studying binding specificities of peptide recognition modules by high-throughput phage display selections.

Simplified synthetic antibody libraries.

MUSI: an integrated system for identifying multiple specificity from very large peptide or nucleic acid data sets.

Charged and hydrophobic surfaces on the a chain of shiga-like toxin 1 recognize the C-terminal domain of ribosomal stalk proteins.

Synthetic antibodies as tools to probe RNA-binding protein function.

Synthetic human monoclonal antibodies toward staphylococcal enterotoxin B (SEB) protective against toxic shock syndrome.

Antibodies for all: The case for genome-wide affinity reagents.

Elucidation of the binding preferences of peptide recognition modules: SH3 and PDZ domains.

Recombinant antibodies and in vitro selection technologies.

Synthetic antibody libraries.

Conformational control of the Ste5 scaffold protein insulates against MAP kinase misactivation.

Selecting and purifying autonomous human variable heavy (VH) domains.

T cell receptor-like recognition of tumor in vivo by synthetic antibody fragment.

Chemical synthesis and X-ray structure of a heterochiral {D-protein antagonist plus vascular endothelial growth factor} protein complex by racemic crystallography.

Superbinder SH2 domains act as antagonists of cell signaling.

A short DNA aptamer that recognizes TNFα and blocks its activity in vitro.

Two synthetic antibodies that recognize and neutralize distinct proteolytic forms of the ebola virus envelope glycoprotein.

Immunology: Evolving superkines for selectivity.

KIF14 negatively regulates Rap1a-Radil signaling during breast cancer progression.

CDR-H3 diversity is not required for antigen recognition by synthetic antibodies.

A strategy for modulation of enzymes in the ubiquitin system.

Engineering and analysis of peptide-recognition domain specificities by phage display and deep sequencing.

Interaction domains of Sos1/Grb2 are finely tuned for cooperative control of embryonic stem cell fate.

Design of synthetic autonomous VH domain libraries and structural analysis of a VH domain bound to vascular endothelial growth factor.

SH3 interactome conserves general function over specific form.

Engineering ubiquitin to modulate the ubiquitin proteosome system.

Targeting HER2+ breast cancer cells: lysosomal accumulation of anti-HER2 antibodies is influenced by antibody binding site and conjugation to polymeric nanoparticles.

Recombinant genetic libraries and human monoclonal antibodies.

Structural basis for KIT receptor tyrosine kinase inhibition by antibodies targeting the D4 membrane-proximal region.

Development of inhibitors in the ubiquitination cascade.

Peptide binding properties of the three PDZ domains of Bazooka (Drosophila Par-3).

Design and generation of synthetic antibody libraries for phage display.

Large-scale interaction profiling of PDZ domains through proteomic peptide-phage display using human and viral phage peptidomes.

Synthetic antibody technologies.

Prediction and experimental characterization of nsSNPs altering human PDZ-binding motifs.

Alteration of the C-terminal ligand specificity of the erbin PDZ domain by allosteric mutational effects.

RPRD1A and RPRD1B are human RNA polymerase II C-terminal domain scaffolds for Ser5 dephosphorylation.

Visualization of arrestin recruitment by a G-protein-coupled receptor.

Synthetic antibodies with a human framework that protect mice from lethal Sudan ebolavirus challenge.

A structural portrait of the PDZ domain family.

A systematic approach to identify novel cancer drug targets using machine learning, inhibitor design and high-throughput screening.

The demonstration of αKlotho deficiency in human chronic kidney disease with a novel synthetic antibody.

The Cdc15 and Imp2 SH3 domains cooperatively scaffold a network of proteins that redundantly ensure efficient cell division in fission yeast.

MicroPET/CT imaging of patient-derived pancreatic cancer xenografts implanted subcutaneously or orthotopically in NOD-scid mice using (64)Cu-NOTA-panitumumab F(ab')2 fragments.

Brain tumor is a sequence-specific RNA-binding protein that directs maternal mRNA clearance during the Drosophila maternal-to-zygotic transition.

A High Through-put Platform for Recombinant Antibodies to Folded Proteins.

A switchable yeast display/secretion system.

Rapid isolation of peptidic inhibitors of the solute carrier family transporters OATP1B1 and OATP1B3 by cell-based phage display selections.

System-Wide Modulation of HECT E3 Ligases with Selective Ubiquitin Variant Probes.

Inhibition of SCF ubiquitin ligases by engineered ubiquitin variants that target the Cul1 binding site on the Skp1-F-box interface.

Saturation scanning of ubiquitin variants reveals a common hot spot for binding to USP2 and USP21.

Engineering cell signaling modulators from native protein-protein interactions.

A Highly Diverse and Functional Naïve Ubiquitin Variant Library for Generation of Intracellular Affinity Reagents.

Creation of Phosphotyrosine Superbinders by Directed Evolution of an SH2 Domain.

Fc Engineering for Developing Therapeutic Bispecific Antibodies and Novel Scaffolds.

Generation and Validation of Intracellular Ubiquitin Variant Inhibitors for USP7 and USP10.

Comprehensive Analysis of the Human SH3 Domain Family Reveals a Wide Variety of Non-canonical Specificities.

A General Strategy for Discovery of Inhibitors and Activators of RING and U-box E3 Ligases with Ubiquitin Variants.

Construction of Synthetic Antibody Phage-Display Libraries.