Anthony Kossiakoff

Structure of a phage display-derived variant of human growth hormone complexed to two copies of the extracellular domain of its receptor: evidence for strong structural coupling between receptor binding sites.

Functional promiscuity of squirrel monkey growth hormone receptor toward both primate and nonprimate growth hormones.

Determination of the energetics governing the regulatory step in growth hormone-induced receptor homodimerization.

The first semi-synthetic serine protease made by native chemical ligation.

Site2 binding energetics of the regulatory step of growth hormone-induced receptor homodimerization.

The functional binding epitope of a high affinity variant of human growth hormone mapped by shotgun alanine-scanning mutagenesis: insights into the mechanisms responsible for improved affinity.

Dissecting the binding energy epitope of a high-affinity variant of human growth hormone: cooperative and additive effects from combining mutations from independently selected phage display mutagenesis libraries.

The structural basis for biological signaling, regulation, and specificity in the growth hormone-prolactin system of hormones and receptors.

The high- and low-affinity receptor binding sites of growth hormone are allosterically coupled.

The crystal structure of a quercetin 2,3-dioxygenase from Bacillus subtilis suggests modulation of enzyme activity by a change in the metal ion at the active site(s).

Intramolecular cooperativity in a protein binding site assessed by combinatorial shotgun scanning mutagenesis.

Total chemical synthesis and X-ray crystal structure of a protein diastereomer: [D-Gln 35]ubiquitin.

Shotgun alanine scanning shows that growth hormone can bind productively to its receptor through a drastically minimized interface.

Alternative views of functional protein binding epitopes obtained by combinatorial shotgun scanning mutagenesis.

The crystal structure of Aq_328 from the hyperthermophilic bacteria Aquifex aeolicus shows an ancestral histone fold.

Dissecting the energetics of protein alpha-helix C-cap termination through chemical protein synthesis.

Crystal structure and site 1 binding energetics of human placental lactogen.

Structure of bistramide A-actin complex at a 1.35 angstroms resolution.

Comprehensive and quantitative mapping of energy landscapes for protein-protein interactions by rapid combinatorial scanning.

The role of protein dynamics in increasing binding affinity for an engineered protein-protein interaction established by H/D exchange mass spectrometry.

Time-controlled microfluidic seeding in nL-volume droplets to separate nucleation and growth stages of protein crystallization.

Exploring and designing protein function with restricted diversity.

High-throughput generation of synthetic antibodies from highly functional minimalist phage-displayed libraries.

Exploring the capacity of minimalist protein interfaces: interface energetics and affinity maturation to picomolar KD of a single-domain antibody with a flat paratope.

Synthetic antibodies for specific recognition and crystallization of structured RNA.

The 1.38 A crystal structure of DmsD protein from Salmonella typhimurium, a proofreading chaperone on the Tat pathway.

Toward chaperone-assisted crystallography: protein engineering enhancement of crystal packing and X-ray phasing capabilities of a camelid single-domain antibody (VHH) scaffold.

X-ray structure of snow flea antifreeze protein determined by racemic crystallization of synthetic protein enantiomers.

Understanding mechanisms governing protein-protein interactions from synthetic binding interfaces.

X-ray structure of native scorpion toxin BmBKTx1 by racemic protein crystallography using direct methods.

Principal determinants leading to transition state formation of a protein-protein complex, orientation trumps side-chain interactions.

Crystal structure of full-length KcsA in its closed conformation.

Racemic crystallography of synthetic protein enantiomers used to determine the X-ray structure of plectasin by direct methods.

An engineered substance P variant for receptor-mediated delivery of synthetic antibodies into tumor cells.

Role of a salt bridge in the model protein crambin explored by chemical protein synthesis: X-ray structure of a unique protein analogue, [V15A]crambin-alpha-carboxamide.

Keeping signaling in check.

Characterization of engineered actin binding proteins that control filament assembly and structure.

A portable RNA sequence whose recognition by a synthetic antibody facilitates structural determination.

Allosteric control of ligand-binding affinity using engineered conformation-specific effector proteins.

Mechanism of activation gating in the full-length KcsA K+ channel.

Substance P derivatives as versatile tools for specific delivery of various types of biomolecular cargo.

Generating conformation-specific synthetic antibodies to trap proteins in selected functional states.

Structure of active β-arrestin-1 bound to a G-protein-coupled receptor phosphopeptide.

Structural mechanism of voltage-dependent gating in an isolated voltage-sensing domain.

Cell biology. New tricks for an old dimer.

Human-chromatin-related protein interactions identify a demethylase complex required for chromosome segregation.

Visualization of arrestin recruitment by a G-protein-coupled receptor.

Probing the functions of the paramyxovirus glycoproteins F and HN with a panel of synthetic antibodies.

Applications for an engineered Protein-G variant with a pH controllable affinity to antibody fragments.

Engineering synthetic antibody binders for allosteric inhibition of prolactin receptor signaling.