David Piston

Reduction in pancreatic transcription factor PDX-1 impairs glucose-stimulated insulin secretion.

Regulation of corepressor function by nuclear NADH.

The efficiency of two-photon photolysis of a "caged" fluorophore, o-1-(2-nitrophenyl)ethylpyranine, in relation to photodamage of synaptic terminals.

Pancreatic islet beta-cells transiently metabolize pyruvate.

A functional link between glucokinase binding to insulin granules and conformational alterations in response to glucose and insulin.

Bioluminescence resonance energy transfer assays for protein-protein interactions in living cells.

Control of glucose phosphorylation in L6 myotubes by compartmentalization, hexokinase, and glucose transport.

Phase sensitive demodulation in multiphoton microscopy.

Bioluminescence resonance energy transfer: monitoring protein-protein interactions in living cells.

Regulation of beta cell glucokinase by S-nitrosylation and association with nitric oxide synthase.

Intrasequence GFP in class I MHC molecules, a rigid probe for fluorescence anisotropy measurements of the membrane environment.

Chemically resolved imaging of biological cells and thin films by infrared scanning near-field optical microscopy.

Development of a novel fluorogenic proteolytic beacon for in vivo detection and imaging of tumour-associated matrix metalloproteinase-7 activity.

Oxidative stress is a mediator of glucose toxicity in insulin-secreting pancreatic islet cell lines.

Noninvasive two-photon imaging reveals retinyl ester storage structures in the eye.

An improved cyan fluorescent protein variant useful for FRET.

Quantitative NAD(P)H/flavoprotein autofluorescence imaging reveals metabolic mechanisms of pancreatic islet pyruvate response.

Nutrient-stimulated insulin secretion in mouse islets is critically dependent on intracellular pH.

Microfluidic glucose stimulation reveals limited coordination of intracellular Ca2+ activity oscillations in pancreatic islets.

High-contrast imaging of fluorescent protein FRET by fluorescence polarization microscopy.

Substrate binding stoichiometry and kinetics of the norepinephrine transporter.

Imaging Beta cell development in real-time using pancreatic explants from mice with green fluorescent protein-labeled pancreatic Beta cells.

When two is better than one: elements of intravital microscopy.

Impaired glucagon secretory responses in mice lacking the type 1 sulfonylurea receptor.

Optical imaging of pancreatic beta cells in living mice expressing a mouse insulin I promoter-firefly luciferase transgene.

Regulation of two insulin granule populations within the reserve pool by distinct calcium sources.

Amiloride derivatives enhance insulin release in pancreatic islets from diabetic mice.

Imaging pancreatic beta-cells in the intact pancreas.

Critical role of gap junction coupled KATP channel activity for regulated insulin secretion.

Mechanisms of time-dependent potentiation of insulin release: involvement of nitric oxide synthase.

Redox sensor CtBP mediates hypoxia-induced tumor cell migration.

The coming of age of two-photon excitation imaging for intravital microscopy.

Metabolic regulation of SIRT1 transcription via a HIC1:CtBP corepressor complex.

Optimization of pairings and detection conditions for measurement of FRET between cyan and yellow fluorescent proteins.

Naked2 acts as a cargo recognition and targeting protein to ensure proper delivery and fusion of TGF-alpha containing exocytic vesicles at the lower lateral membrane of polarized MDCK cells.

Direct effect of cholesterol on insulin secretion: a novel mechanism for pancreatic beta-cell dysfunction.

X-ray structure of Cerulean GFP: a tryptophan-based chromophore useful for fluorescence lifetime imaging.

Leptin requires canonical migratory signaling pathways for induction of monocyte and macrophage chemotaxis.

Fluorescent protein FRET: the good, the bad and the ugly.

FRET by fluorescence polarization microscopy.

Two-photon excitation microscopy for the study of living cells and tissues.

Optical lock-in detection of FRET using synthetic and genetically encoded optical switches.

Dimethyl amiloride improves glucose homeostasis in mouse models of type 2 diabetes.

Kinetics of a collagen-like polypeptide fragmentation after mid-IR free-electron laser ablation.

Turning fluorescent proteins into energy-saving light bulbs.

Establishment and quantitative imaging of a 3D lung organotypic model of mammary tumor outgrowth.

Gap junction coupling and calcium waves in the pancreatic islet.

Real-time, multidimensional in vivo imaging used to investigate blood flow in mouse pancreatic islets.

Subcutaneous transplantation of embryonic pancreas for correction of type 1 diabetes.

Single-photon-counting detector for increased sensitivity in two-photon laser scanning microscopy.

Chapter 4: Combining microfluidics and quantitative fluorescence microscopy to examine pancreatic islet molecular physiology.

Rapid and inexpensive fabrication of polymeric microfluidic devices via toner transfer masking.

Photoconversion in orange and red fluorescent proteins.

Deletion of the mouse Slc30a8 gene encoding zinc transporter-8 results in impaired insulin secretion.

Flecainide inhibits arrhythmogenic Ca2+ waves by open state block of ryanodine receptor Ca2+ release channels and reduction of Ca2+ spark mass.

Quantitative measurement of zinc secretion from pancreatic islets with high temporal resolution using droplet-based microfluidics.

Glucose-dependent blood flow dynamics in murine pancreatic islets in vivo.

Fluorescent protein tracking and detection: fluorescent protein structure and color variants.

Fluorescent protein tracking and detection: applications using fluorescent proteins in living cells.

Glucose suppression of glucagon secretion: metabolic and calcium responses from alpha-cells in intact mouse pancreatic islets.

Selective visualization of cyclooxygenase-2 in inflammation and cancer by targeted fluorescent imaging agents.

Gαo represses insulin secretion by reducing vesicular docking in pancreatic beta-cells.

Rapid extravasation and establishment of breast cancer micrometastases in the liver microenvironment.

Fluorescence anisotropy of protein complexes in living cells.

Noninvasive multiphoton fluorescence microscopy resolves retinol and retinal condensation products in mouse eyes.

Fluorescent proteins at a glance.

Quantification of factors influencing fluorescent protein expression using RMCE to generate an allelic series in the ROSA26 locus in mice.

Amphiregulin exosomes increase cancer cell invasion.

Raman-shifted alexandrite laser for soft tissue ablation in the 6- to 7-µm wavelength range.

Gap junctions and other mechanisms of cell-cell communication regulate basal insulin secretion in the pancreatic islet.

Reversal of Type 1 Diabetes in Mice by Brown Adipose Tissue Transplant.