detection-phytophthora-capsici-irrigation-water-using-loop-mediated

Detection of <em>Phytophthora capsici</em> in Irrigation Water using Loop-Mediated Isothermal Amplification

Phytophthora capsici is a devastating oomycete pathogen that affects many important solanaceous and cucurbit crops causing significant economic losses in ...

Tennessee State University

Flooding can increase tree susceptibility to root rot pathogens as well as attacks by ambrosia beetles attracted to stress-induced ethanol emissions. The objective of this study was to investigate the interaction of a preventative fungicide treatment and root infection with  on ambrosia beetle attacks in flood stressed trees. A fungicide (Pageant Intrinsic) was evaluated in two flood trials using Eastern redbud and tulip poplar trees with treatments including the fungicide with or without pathogen or no fungicide with or without pathogen. Fungicide treated trees had fewer ambrosia beetle attacks, particularly in trees without  co-infection. In a follow-up experiment, ethanol content was evaluated in flooded redbuds to determine if the fungicide treatment reduced stress-induced compounds. All flood stressed trees began producing ethanol within 24 h post flooding, regardless of fungicide treatment or  infection. We conclude that pre-treatments of a fungicide can provide protection from ambrosia beetle attacks during an extreme flood event, but that protection is reduced if a root rot pathogen is also present. Additionally, rejection of fungicide treated trees was not related to the absence of ethanol, as the fungicide-treated plants released ethanol in quantities similar to non-treated trees.

Interaction of a Preventative Fungicide Treatment and Root Rot Pathogen on Ambrosia Beetle Attacks during a Simulated Flood Event.

During flooding events in nurseries, Phytophthora root rot caused by  Rands often causes damage that leads to complete crop loss. In this study, we evaluated the efficacy of fungicides, biofungicides, and host plant defense inducers for preventive and curative control of Phytophthora root rot on flowering dogwood ( L.) seedlings exposed to a simulated flood event of 1, 3, or 7 days. In two greenhouse trials, preventive (7 days before flooding) or curative (1 day after flooding) drench treatments were applied to dogwood seedlings artificially inoculated with . . The plants were flooded by maintaining standing water for 1, 3, or 7 days. After the trials, plant growth data (total plant weight, root weight, plant height, and plant width) were recorded, and root systems were assessed for disease severity using a scale of 0 to 100% of roots affected, and subsamples were plated on PARPH-V8 medium to determine the percent recovery of the  pathogen. Plants preventively treated with Subdue MAXX had reduced disease severity relative to the nontreated, inoculated plants (positive control) flooded 1, 3, or 7 days in both trials. Pageant Intrinsic and Segovis treatments also had lower disease severity than the positive control at all flooding durations in trial two, but not trial one. In trial one, preventive and curative treatments of Orkestra Intrinsic had reduced disease severity compared with the positive control at 1 and 3 days of flooding, whereas curative treatments of Empress Intrinsic and Tartan Stressgard also were effective at 1 and 3 days of flooding in trial one. The host plant defense inducers (Aliette 80 WDG, Signature Xtra, and Actigard) were inconsistent and ineffective at reducing disease severity when applied as preventive or curative treatments. Preventive treatments of the biofungicides RootShield Plus and MBI-110 had consistently lower disease severity than the positive control at 1 day of flooding but not 3 or 7 days of flooding. Potentially, growers can use information from this study to manage Phytophthora root rot during flooding or in areas of the nursery that often experience high soil moisture levels.

Comparative Performance of Fungicides, Biofungicides, and Host Plant Defense Inducers in Suppression of Phytophthora Root Rot in Flowering Dogwood During Simulated Root Flooding Events.

Soilborne diseases are the most economically significant problem faced by Southern region nursery producers. The goal of this research was to improve Rhizoctonia root rot disease management through the use of soil solarization alone and in combination with biofumigant cover crops-arugula 'Astro' ( ssp. ), mustard green 'Amara' (), and turnip 'Purple top forage' (); good quality compost and mustard meal amendment. The experiments were established as on-farm trials in 2016 and 2017 with prevalent  population in propagation beds. All three biofumigant cover crops, arugula 'Astro', mustard green 'Amara', and turnip 'Purple top forage' in combination with solarization were able to reduce the Rhizoctonia root rot in flowering cherry 'Kwanzan' plants in nursery propagation beds. Compost amendment increased the flowering cherry rooted cuttings growth (plant weight, root weight, and plant height) compared to other treatments. Soil solarization in combination with cover crops and organic inputs could be used as part of an integrated approach to manage Rhizoctonia root rot in nursery crop propagation beds.

Effect of Organic Inputs and Solarization for the Suppression of  in Woody Ornamental Plant Production.

Ornamental plants in the genus  are extensively planted in landscapes and home gardens around the world. A major limitation to a more widespread use of these plants is their susceptibility to powdery mildew (PM). In this study, we used multilocus sequence typing (MLST) analysis to gain insights into the population diversity of 32  PM pathogen ( and  sp.) isolates collected from the eastern United States and relate it to the ability to overcome host resistance. Low genetic diversity and a lack of structure were found within our population. Whole genome comparison of two isolates was used to support low genetic diversity evidence found with the MLST analysis. Recombination was suggested by the incongruences observed in the six phylogenetic trees generated from the housekeeping genes TEF-1α, CSI, ITS, IGS, H3, and TUB. Contrasting with low genetic diversity, we found high phenotypic diversity when using 10 of the 32 isolates to evaluate host resistance in four different  species ( 'Dunbar Creek',  OPGC 3598,  OPGC 3594, and  OPGC 4185) using in vitro bioassays. We observed quantitative and qualitative resistance in all  species and a consistent low disease severity in our control,  'Dunbar Creek'. Taken together, the results generated in this study constitute a robust screening of popular  germplasm that can be incorporated into breeding programs for PM resistance and provides significant information on the evolution of PM pathogens.

Fulya Baysal-Gurel

Department of Agricultural and Environmental Sciences,
Otis L. Floyd Nursery Research Center,
Department of Agricultural and Environmental Sciences, Otis L. Floyd Nursery Research Center

Tennessee State University

Detection of Phytophthora capsici in Irrigation Water using Loop-Mediated Isothermal Amplification

Fulya Baysal-Gurel

Department of Agricultural and Environmental Sciences,Otis L. Floyd Nursery Research Center,Department of Agricultural and Environmental Sciences, Otis L. Floyd Nursery Research Center

Tennessee State University

Detection of Phytophthora capsici in Irrigation Water using Loop-Mediated Isothermal Amplification

Department of Agricultural and Environmental Sciences,
Otis L. Floyd Nursery Research Center,
Department of Agricultural and Environmental Sciences, Otis L. Floyd Nursery Research Center