particle-templated-emulsification-enables-microfluidic-free-droplet

Particle Templated Emulsification enables Microfluidic-Free Droplet Assays

Reactions performed in monodispersed droplets afford enhanced accuracy and sensitivity compared to equivalent ones performed in bulk. However, the ...

University of California

The compartmentalization of reactions in monodispersed droplets is valuable for applications across biology. However, the requirement of microfluidics to partition the sample into monodispersed droplets is a significant barrier that impedes implementation. Here, we introduce particle-templated emulsification, a method to encapsulate samples in monodispersed emulsions without microfluidics. By vortexing a mixture of hydrogel particles and sample solution, we encapsulate the sample in monodispersed emulsions that are useful for most droplet applications. We illustrate the method with ddPCR and single cell culture. The ability to encapsulate samples in monodispersed droplets without microfluidics should facilitate the implementation of compartmentalized reactions in biology.

Makiko N. Hatori

Department of Bioengineering and Therapeutic Sciences,
California Institute for Quantitative Biosciences,
Department of Bioengineering and Therapeutic Sciences, California Institute for Quantitative Biosciences

University of California

Particle Templated Emulsification enables Microfluidic-Free Droplet Assays

Makiko N. Hatori

.css-o250i3{font-size:18px;font-family:Open Sans,sans-serif;line-height:21.6px;}Department of Bioengineering and Therapeutic Sciences,California Institute for Quantitative Biosciences,Department of Bioengineering and Therapeutic Sciences, California Institute for Quantitative Biosciences

University of California

Particle Templated Emulsification enables Microfluidic-Free Droplet Assays

Department of Bioengineering and Therapeutic Sciences,
California Institute for Quantitative Biosciences,
Department of Bioengineering and Therapeutic Sciences, California Institute for Quantitative Biosciences