Masataka Kinjo

Novel fluorescence labeling and high-throughput assay technologies for in vitro analysis of protein interactions.

In situ observation of mobility and anchoring of PKCbetaI in plasma membrane.

Protein folding by the effects of macromolecular crowding.

Systematic error in fluorescence correlation measurements identified by a simple saturation model of fluorescence.

DNA measurements by using fluorescence correlation spectroscopy and two-color fluorescence cross correlation spectroscopy.

Direct detection of caspase-3 activation in single live cells by cross-correlation analysis.

Molecular dynamics of STAT3 on IL-6 signaling pathway in living cells.

Calixarene-coated water-soluble CdSe-ZnS semiconductor quantum dots that are highly fluorescent and stable in aqueous solution.

Dead-time distortion in fluorescence correlation measurements.

Amphiphilic p-sulfonatocalix[4]arene-coated CdSe/ZnS quantum dots for the optical detection of the neurotransmitter acetylcholine.

Control of the optical properties of quantum dots by surface coating with calix[n]arene carboxylic acids.

Fluorescence cross-correlation analyses of the molecular interaction between an Aux/IAA protein, MSG2/IAA19, and protein-protein interaction domains of auxin response factors of arabidopsis expressed in HeLa cells.

Lateral mobility of membrane-binding proteins in living cells measured by total internal reflection fluorescence correlation spectroscopy.

Microenvironment and effect of energy depletion in the nucleus analyzed by mobility of multiple oligomeric EGFPs.

The regulator of the F1 motor: inhibition of rotation of cyanobacterial F1-ATPase by the epsilon subunit.

Analysis of intranuclear binding process of glucocorticoid receptor using fluorescence correlation spectroscopy.

Diffusion analysis of glucocorticoid receptor and antagonist effect in living cell nucleus.

The novel cargo Alcadein induces vesicle association of kinesin-1 motor components and activates axonal transport.

Specificity and similarity of functions of the Aux/IAA genes in auxin signaling of Arabidopsis revealed by promoter-exchange experiments among MSG2/IAA19, AXR2/IAA7, and SLR/IAA14.

DNA microstructure based on self-assembly of 4-sticky-end Holliday junctions in aqueous solution.

Detection of polyglutamine protein oligomers in cells by fluorescence correlation spectroscopy.

Dissociation of the insulin receptor and caveolin-1 complex by ganglioside GM3 in the state of insulin resistance.

Detection of prion protein immune complex for bovine spongiform encephalopathy diagnosis using fluorescence correlation spectroscopy and fluorescence cross-correlation spectroscopy.

Preparation and characterization of thiacalix[4]arene coated water-soluble CdSe/ZnS quantum dots as a fluorescent probe for Cu2+ ions.

Detection of antigen protein by using fluorescence cross-correlation spectroscopy and quantum-dot-labeled antibodies.

Analysis of the molecular dynamics of medaka nuage proteins by fluorescence correlation spectroscopy and fluorescence recovery after photobleaching.

Regulated motion of glycoproteins revealed by direct visualization of a single cargo in the endoplasmic reticulum.

Application of fluorescence lifetime imaging of enhanced green fluorescent protein to intracellular pH measurements.

Stress-induced environmental changes in a single cell as revealed by fluorescence lifetime imaging.

Reciprocal interaction with G-actin and tropomyosin is essential for aquaporin-2 trafficking.

Fluorescence cross-correlation spectroscopy of plant proteins.

The A- and B-type nuclear lamin networks: microdomains involved in chromatin organization and transcription.

Multipoint fluorescence correlation spectroscopy with total internal reflection fluorescence microscope.

Fluorescence lifetime images of green fluorescent protein in HeLa cells during TNF-alpha induced apoptosis.

[Cell function analysis by fluorescence fluctuation spectroscopy].

Confomational analysis of soluble oligomers of GFP tagged prion protein by fluorescence fluctuation spectroscopy.

Monitoring intracellular degradation of exogenous DNA using diffusion properties.

Kinesin-1/Hsc70-dependent mechanism of slow axonal transport and its relation to fast axonal transport.

Single-cell quantitative analysis of DNA incorporation and protein expression in microwells.

Low pH-triggered model drug molecule release from virus-like particles.

In vivo evidence for the fibrillar structures of Sup35 prions in yeast cells.

STPR, a 23-amino acid tandem repeat domain, found in the human function-unknown protein ZNF821.

Dynamic assembly properties of nonmuscle myosin II isoforms revealed by combination of fluorescence correlation spectroscopy and fluorescence cross-correlation spectroscopy.

Monitoring the caspase cascade in single apoptotic cells using a three-color fluorescent protein substrate.

Two distinct amyloid beta-protein (Abeta) assembly pathways leading to oligomers and fibrils identified by combined fluorescence correlation spectroscopy, morphology, and toxicity analyses.

[Study of protein function using fluorescence correlation spectroscopy].

Direct observation of dimerization between different CREB1 isoforms in a living cell.

Importance of sialic acid residues illuminated by live animal imaging using phosphorylcholine self-assembled monolayer-coated quantum dots.

Intracellular pH sensing using autofluorescence lifetime microscopy.

A modified FCCS procedure applied to Ly49A-MHC class I cis-interaction studies in cell membranes.

Current research on protein-protein interactions among auxin-signaling factors in regulation of plant growth and development.

A small peptide sequence is sufficient for initiating kinesin-1 activation through part of TPR region of KLC1.

Atg9 vesicles are an important membrane source during early steps of autophagosome formation.

Direct association of unfolded proteins with mammalian ER stress sensor, IRE1β.

Nonmuscle myosin II folds into a 10S form via two portions of tail for dynamic subcellular localization.

pH dependence of the fluorescence lifetime of FAD in solution and in cells.

[Fluorescence cross-correlation spectroscopy for molecular fluorescence cross-correlation spectroscopy for molecular dynamics in the living cell].

Virus-like particles with removable cyclodextrins enable glutathione-triggered drug release in cells.

Siglec-15 regulates osteoclast differentiation by modulating RANKL-induced phosphatidylinositol 3-kinase/Akt and Erk pathways in association with signaling Adaptor DAP12.

Prefoldin protects neuronal cells from polyglutamine toxicity by preventing aggregation formation.

Determination of dissociation constant of the NFκB p50/p65 heterodimer using fluorescence cross-correlation spectroscopy in the living cell.

Simple and direct assembly of kymographs from movies using KYMOMAKER.

A rapid and high-throughput quantitation assay of the nuclear factor κB activity using fluorescence correlation spectroscopy in the setting of clinical laboratories.

The interaction of Hsp104 with yeast prion Sup35 as analyzed by fluorescence cross-correlation spectroscopy.

Dysregulation of the proteasome increases the toxicity of ALS-linked mutant SOD1.

Moyamoya disease-associated protein mysterin/RNF213 is a novel AAA+ ATPase, which dynamically changes its oligomeric state.

Quantitative analysis of APP axonal transport in neurons: role of JIP1 in enhanced APP anterograde transport.

Siglec-15 is a potential therapeutic target for postmenopausal osteoporosis.

Conformational analysis of misfolded protein aggregation by FRET and live-cell imaging techniques.

Efficient and dynamic nuclear localization of green fluorescent protein via RNA binding.

Screening for FtsZ Dimerization Inhibitors Using Fluorescence Cross-Correlation Spectroscopy and Surface Resonance Plasmon Analysis.

Homodimerization of glucocorticoid receptor from single cells investigated using fluorescence correlation spectroscopy and microwells.

Raster image cross-correlation analysis for spatiotemporal visualization of intracellular degradation activities against exogenous DNAs.

Interaction of RNA with a C-terminal fragment of the amyotrophic lateral sclerosis-associated TDP43 reduces cytotoxicity.

Dependence of fluorescent protein brightness on protein concentration in solution and enhancement of it.

Development of new fusion proteins for visualizing amyloid-β oligomers in vivo.

In vivo fluorescence correlation spectroscopy analyses of FMBP-1, a silkworm transcription factor.

Polarization-dependent fluorescence correlation spectroscopy for studying structural properties of proteins in living cell.

Temperature and pH sensitivity of a stabilized self-nanoemulsion formed using an ionizable lipid-like material via an oil-to-surfactant transition.

Different aggregation states of a nuclear localization signal-tagged 25-kDa C-terminal fragment of TAR RNA/DNA-binding protein 43 kDa.

A Quantitative Study of Internal and External Interactions of Homodimeric Glucocorticoid Receptor Using Fluorescence Cross-Correlation Spectroscopy in a Live Cell.

Negative Correlation between the Diffusion Coefficient and Transcriptional Activity of the Glucocorticoid Receptor.

Phosphorylation of multiple sites within an acidic region of Alcadein α is required for kinesin-1 association and Golgi exit of Alcadein α cargo.

Phosphorylation of KLC1 modifies interaction with JIP1 and abolishes the enhanced fast velocity of APP transport by kinesin-1.

U6 snRNA expression prevents toxicity in TDP-43-knockdown cells.

Not Oligomers but Amyloids are Cytotoxic in the Membrane-Mediated Amyloidogenesis of Amyloid-β Peptides.

Physicochemical Properties of the Mammalian Molecular Chaperone HSP60.

Detection of substrate binding of a collagen-specific molecular chaperone HSP47 in solution using fluorescence correlation spectroscopy.

Determination of diffusion coefficients in live cells using fluorescence recovery after photobleaching with wide-field fluorescence microscopy.

State-of-the-Art Fluorescence Fluctuation-Based Spectroscopic Techniques for the Study of Protein Aggregation.

Two-detector number and brightness analysis reveals spatio-temporal oligomerization of proteins in living cells.

Determination of cytoplasmic optineurin foci sizes using image correlation spectroscopy.

Relationship Between Homodimeric Glucocorticoid Receptor and Transcriptional Regulation Assessed via an In Vitro Fluorescence Correlation Spectroscopy-Microwell System.

Analysis of the substrate recognition state of TDP-43 to single-stranded DNA using fluorescence correlation spectroscopy.

The cytoplasmic region of the amyloid β-protein precursor (APP) is necessary and sufficient for the enhanced fast velocity of APP transport by kinesin-1.

Molecular chaperone HSP70 prevents formation of inclusion bodies of the 25-kDa C-terminal fragment of TDP-43 by preventing aggregate accumulation.

Absolute Quantification of RNA Molecules Using Fluorescence Correlation Spectroscopy with Certified Reference Materials.

Encapsulation of biomacromolecules by soaking and co-crystallization into porous protein crystals of hemocyanin.

Characterization of Intracellular Crowding Environments with Topology-Based DNA Quadruplex Sensors.

Multipoint fluorescence correlation spectroscopy using spatial light modulator.

Functional Fluorescence Microscopy Imaging: Quantitative Scanning-Free Confocal Fluorescence Microscopy for the Characterization of Fast Dynamic Processes in Live Cells.

Quantitative Analysis of Membrane Surface and Small Confinement Effects on Molecular Diffusion.

Biophysical research in Hokkaido University, Japan.

Membrane Surface Modulates Slow Diffusion in Small Crowded Droplets.

Empirical Bayes method using surrounding pixel information for number and brightness analysis.

Number and Brightness Analysis: Visualization of Protein Oligomeric State in Living Cells.

Molecular basis of functional exchangeability between ezrin and other actin-membrane associated proteins during cytokinesis.