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Contact Mode Atomic Force Microscopy as a Rapid Technique for Morphological Observation and Bacterial Cell Damage Analysis

Electron microscopy is one of the tools required to characterize cellular structures. However, the procedure is complicated and expensive due to the ...

Universidad de Guadalajara

The morphology and elemental composition of crystals in embryos, seedlings and adult plants of the globose cacti Mammillaria uncinata were studied. Samples of mature fruits and adult plants were collected. To obtain embryos and seedlings, seeds from mature fruits were germinated under laboratory conditions. Both embryos and seedlings as well as stem and root of the adult plants were processed by conventional microtechniques and tissue macerations to isolate the individual crystals. The crystal morphology was evaluated by light and scanning electron microscopy while its chemical composition was analyzed by energy dispersive spectroscopy, IR and Raman spectroscopy. The results demonstrated the occurrence of calcium oxalate crystals in the three stages of plant growth. Solitary and small crystals are present in embryos and seedlings in contrast crystals are aggregates or conglomerates in the adult plants. The compositional analysis showed that seedling crystals contain carbon (50.37%), oxygen (45.29%) and calcium (3.36%) while in adult plants the percentage has changed to carbon (12.54%), oxygen (53.06%) and calcium (34.38%). In the IR spectrum, the vibration bands around 1321 and 1621 cm are attributed to the calcium oxalate in the dihydrate state (weddellite), the Raman peak at 1475 cm shows also that crystals correspond to the pure state of calcium oxalate dihydrate state. The crystal size was also different for seedlings and adult plants, mean values varied from 12.11 to 13.38 μm for width and length, respectively in seedlings and from 65.10 to 73.90 μm, in adult plants. It is concluded that the elemental composition, size and morphology of crystals in M. uncinata depend on the growth stage, as it happens in other plant species.

Variability and composition of calcium oxalate crystals in embryos-seedlings-adult plants of the globose cacti Mammillaria uncinata.

Magnesium oxide nanoparticles (MgO NPs) were obtained by the calcination of precursor microparticles (PM) synthesized by a novel triethylamine-based precipitation method. Scanning electron microscopy (SEM) revealed a mean size of 120 nm for the MgO NPs. The results of the characterizations for MgO NPs support the suggestion that our material has the capacity to attack, and have an antibacterial effect against, Gram-negative and Gram-positive bacteria strains. The ability of the MgO NPs to produce reactive oxygen species (ROS), such as superoxide anion radicals (O2•-) or hydrogen peroxide (HO), was demonstrated by the corresponding quantitative assays. The MgO antibacterial activity was evaluated against Gram-positive  and Gram-negative  bacteria, with minimum inhibitory concentrations (MICs) of 250 and 500 ppm on the microdilution assays, respectively. Structural changes in the bacteria, such as membrane collapse; surface changes, such as vesicular formation; and changes in the longitudinal and horizontal sizes, as well as the circumference, were observed using atomic force microscopy (AFM). The lipidic peroxidation of the bacterial membranes was quantified, and finally, a bactericidal mechanism for the MgO NPs was also proposed.

Two-Step Triethylamine-Based Synthesis of MgO Nanoparticles and Their Antibacterial Effect against Pathogenic Bacteria.

Amino acid complexes of zirconia represent an entirely new class of materials that were synthesized and studied for the first time for the decontamination of fluoride ion containing aqueous solutions. Glutamic and aspartic acid complexes of zirconia assembled with thin carbon (stacked graphene oxide) platelets deriving from graphite oxide (GO) were synthesized by a two-step method to prepare adsorbents. The characterization of the complexes was carried out using infrared spectroscopy to determine the functional groups and the types of interaction between the composites and fluoride ions. To reveal the mechanisms and extent of adsorption, two types of batch adsorption measurements were performed: (i) varying equilibrium fluoride ion concentrations to construct adsorption isotherms at pH = 7 in the absence of added electrolytes and (ii) using fixed initial fluoride ion concentrations (10 mg/L) with a variation of either the pH or the concentration of a series of salts that potentially interfere with adsorption. The experimental adsorption isotherms were fitted by three different theoretical isotherm equations, and they are described most appropriately by the two-site Langmuir model for both adsorbents. The adsorption capacities of Zr-glutamic acid-graphite oxide and Zr-aspartic acid-graphite oxide are 105.3 and 101.0 mg/g, respectively. We found that two distinct binding modes are combined in the Zr-amino acid complexes: at low solution concentrations, F ions are preferentially adsorbed by coordinating to the surface Zr species up to a capacity of ca. 10 mg/g. At higher concentrations, however, large amounts of fluoride ions may undergo anion exchange processes and physisorption may occur on the positively charged ammonium moieties of the interfacially bound amino acid molecules. The high adsorption capacity and affinity of the studied dicarboxylate-type amino acids demonstrate that amino acid complexes of zirconia are highly variable materials for the safe and efficient capture of strong Lewis base-type ions such as fluoride.

Amino Acid Complexes of Zirconium in a Carbon Composite for the Efficient Removal of Fluoride Ions from Water.

This study reports a green, simple, and fast method for the synthesis of gold and silver nanoparticles using natural antioxidant compounds. The aqueous extract from dried rosehips (pseudofruit of  L.) was used as a reducing and capping agent of HAuCl and AgNO during the noble metal colloid synthesis at room temperature and no other chemical reagent was used. The high antioxidant activity of the plant extract was proven by 2,2-diphenyl-1-picrylhydrazyl assay by a spectrophotometric method. The formation of stable gold and silver nanoparticles was observed by UV-visible spectroscopy and the evolution of their characteristic surface plasmon resonance band was followed over several days. Transmission electron microscopy confirmed the formation of quasi-spherical nanoparticles with mean diameters 26 and 34 nm, for gold and silver nanoparticles, respectively; XRD revealed an FCC crystalline structure for both gold and silver NPs. The effects of concentrations of noble metal precursor and plant extract solution on the formation, stabilization and size of nanoparticles are discussed, as well as some applications of these colloids.

One-pot green synthesis of gold and silver nanoparticles using  L. extract.

Functionalized platinum nanoparticles have been of considerable interest in recent research due to their properties and applications, among which they stand out as therapeutic agents. The functionalization of the surfaces of nanoparticles can overcome the limits of medicine by increasing selectivity and thereby reducing the side effects of conventional drugs. With the constant development of nanotechnology in the biomedical field, functionalized platinum nanoparticles have been used to diagnose and treat diseases such as cancer and infections caused by pathogens. This review reports on physical, chemical, and biological methods of obtaining platinum nanoparticles and the advantages and disadvantages of their synthesis. Additionally, applications in the biomedical field that can be utilized once the surfaces of nanoparticles have been functionalized with different bioactive molecules are discussed, among which antibodies, biodegradable polymers, and biomolecules stand out.

Rita Patakfalvi

Centro Universitario de los Lagos

Universidad de Guadalajara

Contact Mode Atomic Force Microscopy as a Rapid Technique for Morphological Observation and Bacterial Cell Damage Analysis

Rita Patakfalvi

.css-o250i3{font-size:18px;font-family:Open Sans,sans-serif;line-height:21.6px;}Centro Universitario de los Lagos

Universidad de Guadalajara

Contact Mode Atomic Force Microscopy as a Rapid Technique for Morphological Observation and Bacterial Cell Damage Analysis

Centro Universitario de los Lagos