Viacheslav O. Nikolaev

Novel single chain cAMP sensors for receptor-induced signal propagation.

Real-time monitoring of the PDE2 activity of live cells: hormone-stimulated cAMP hydrolysis is faster than hormone-stimulated cAMP synthesis.

Interplay of Ca2+ and cAMP signaling in the insulin-secreting MIN6 beta-cell line.

Turn-on switch in parathyroid hormone receptor by a two-step parathyroid hormone binding mechanism.

Fluorescent sensors for rapid monitoring of intracellular cGMP.

Monitoring of cAMP synthesis and degradation in living cells.

Molecular basis of partial agonism at the neurotransmitter alpha2A-adrenergic receptor and Gi-protein heterotrimer.

Gs activation is time-limiting in initiating receptor-mediated signaling.

Simultaneous optical measurements of cytosolic Ca2+ and cAMP in single cells.

Cyclic AMP imaging in adult cardiac myocytes reveals far-reaching beta1-adrenergic but locally confined beta2-adrenergic receptor-mediated signaling.

Real-time optical recording of beta1-adrenergic receptor activation reveals supersensitivity of the Arg389 variant to carvedilol.

cAMP microdomains and L-type Ca2+ channel regulation in guinea-pig ventricular myocytes.

Live cell monitoring of mu-opioid receptor-mediated G-protein activation reveals strong biological activity of close morphine biosynthetic precursors.

A novel fluorescence method for the rapid detection of functional beta1-adrenergic receptor autoantibodies in heart failure.

Kinetic analysis of G protein-coupled receptor signaling using fluorescence resonance energy transfer in living cells.

Monitoring receptor signaling by intramolecular FRET.

Conformational cross-talk between alpha2A-adrenergic and mu-opioid receptors controls cell signaling.

Optical techniques to analyze real-time activation and signaling of G-protein-coupled receptors.

Spatiotemporal dynamics of beta-adrenergic cAMP signals and L-type Ca2+ channel regulation in adult rat ventricular myocytes: role of phosphodiesterases.

Widespread receptivity to neuropeptide PDF throughout the neuronal circadian clock network of Drosophila revealed by real-time cyclic AMP imaging.

Real-time monitoring of phosphodiesterase inhibition in intact cells.

Cytoplasmic cAMP concentrations in intact cardiac myocytes.

Immunoadsorption in a 40 year old man with dilated cardiomyopathy and underlying active myocarditis.

Multidrug resistance-associated protein 4 regulates cAMP-dependent signaling pathways and controls human and rat SMC proliferation.

Alternative splicing of the guanylyl cyclase-A receptor modulates atrial natriuretic peptide signaling.

Novel techniques for real-time monitoring of cGMP in living cells.

GPCR and G proteins: drug efficacy and activation in live cells.

Sweet taste receptor expressed in pancreatic beta-cells activates the calcium and cyclic AMP signaling systems and stimulates insulin secretion.

Cyclic GMP from the surrounding somatic cells regulates cyclic AMP and meiosis in the mouse oocyte.

A technique for monitoring multiple signals with a combination of prism-based total internal reflection fluorescence microscopy and epifluorescence microscopy.

Persistent cAMP-signals triggered by internalized G-protein-coupled receptors.

Gq-mediated Ca2+ signals inhibit adenylyl cyclases 5/6 in vascular smooth muscle cells.

Distinct pools of cAMP centre on different isoforms of adenylyl cyclase in pituitary-derived GH3B6 cells.

A fluorescence resonance energy transfer-based M2 muscarinic receptor sensor reveals rapid kinetics of allosteric modulation.

Beta2-adrenergic receptor redistribution in heart failure changes cAMP compartmentation.

Bile acid-induced arrhythmia is mediated by muscarinic M2 receptors in neonatal rat cardiomyocytes.

Signaling by internalized G-protein-coupled receptors.

Imaging of persistent cAMP signaling by internalized G protein-coupled receptors.

Type 4 phosphodiesterase plays different integrating roles in different cellular domains in pyramidal cortical neurons.

Real-time monitoring of somatostatin receptor-cAMP signaling in live pituitary.

Epidermal growth factor receptor kinase activity is required for gap junction closure and for part of the decrease in ovarian follicle cGMP in response to LH.

Scanning ion conductance microscopy: a convergent high-resolution technology for multi-parametric analysis of living cardiovascular cells.

Distinct pharmacological properties of morphine metabolites at G(i)-protein and β-arrestin signaling pathways activated by the human μ-opioid receptor.

Endocytosis in the mouse oocyte and its contribution to cAMP signaling during meiotic arrest.

FRET measurements of intracellular cAMP concentrations and cAMP analog permeability in intact cells.

A protective antiarrhythmic role of ursodeoxycholic acid in an in vitro rat model of the cholestatic fetal heart.

Atrial natriuretic peptide enhances microvascular albumin permeability by the caveolae-mediated transcellular pathway.

A cardiac pathway of cyclic GMP-independent signaling of guanylyl cyclase A, the receptor for atrial natriuretic peptide.

Low angle light scattering analysis: a novel quantitative method for functional characterization of human and murine platelet receptors.

Popeye domain containing proteins are essential for stress-mediated modulation of cardiac pacemaking in mice.

Plasticity of surface structures and β(2)-adrenergic receptor localization in failing ventricular cardiomyocytes during recovery from heart failure.

DT-2 is a specific PKG I inhibitor only in vitro, but not in a living cells.

High Levels of Circulating Epinephrine Trigger Apical Cardiodepression in a β2-Adrenergic Receptor/Gi-Dependent Manner: A New Model of Takotsubo Cardiomyopathy.