Magnetic Fluorescent Bead-Based Dual-Reporter Flow Analysis of PDL1-Vaxx Peptide Vaccine-Induced Antibody Blockade of the PD-1/PD-L1 InteractionJay Overholser *1, Linlin Guo *1, Pravin T. P. Kaumaya 1,2
1Department of Obstetrics & Gynecology, The Ohio State University Wexner Medical Center, 2The James Comprehensive Cancer Center
Checkpoint inhibitors are important targets in developing therapies for the battle against cancer. This report introduces a novel PDL1 peptide-based cancer vaccine, PDL1-Vaxx, which induces neutralizing polyclonal antibody production that blocks PD-1/PDL1 complex formation. This work also details the development and testing of a fluorescent bead-based assay for analyzing this activity.
