Reprogramming Pancreatic Ductal Adenocarcinoma to PluripotencyAmani Alshaikh 1,2, Dmytro Grygoryev 3, Dove Keith 4, Brett Sheppard 4,5, Rosalie C Sears 4,6, Jungsun Kim 3,6, Abdenour Soufi 1
1Centre for Regenerative Medicine, Institute of Regeneration and Repair, Institute of Stem Cell Research, The University of Edinburgh, 2King Abdulaziz City for Science and Technology Health Sector (KACST), 3Cancer Early Detection Advanced Research Center, Knight Cancer Institute, Oregon Health & Science University, 4Brenden-Colson Canter for Pancreatic Care, Oregon Health & Science University, 5Department of Surgery, Oregon Health & Science University, 6Department of Molecular & Medical Genetics, Oregon Health & Science University
The present protocol describes the reprogramming of Pancreatic Ductal Adenocarcinoma (PDAC) and normal pancreatic ductal epithelial cells into induced pluripotent stem cells (iPSCs). We provide an optimized and detailed, step-by-step procedure, from preparing lentivirus to establishing stable iPSC lines.
