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Montefiore Medical Center

2 ARTICLES PUBLISHED IN JoVE

Cancer Research

Assessing Tumor Microenvironment of Metastasis Doorway-Mediated Vascular Permeability Associated with Cancer Cell Dissemination using Intravital Imaging and Fixed Tissue Analysis

George S. Karagiannis^1,2,3, Jessica M. Pastoriza^1,2,4, Lucia Borriello^1,2, Rojin Jafari^1,2, Anouchka Coste^1,2,4, John S. Condeelis^1,2,3,4, Maja H. Oktay^1,2,3,5, David Entenberg^1,2,3

¹Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, ²Gruss-Lipper Biophotonics Center, Albert Einstein College of Medicine, ³Integrated Imaging Program, Albert Einstein College of Medicine, ⁴Department of Surgery, Montefiore Medical Center, ⁵Department of Pathology, Montefiore Medical Center

We describe two methods for assessing transient vascular permeability associated with tumor microenvironment of metastasis (TMEM) doorway function and cancer cell intravasation using intravenous injection of high-molecular weight (155 kDa) dextran in mice. The methods include intravital imaging in live animals and fixed tissue analysis using immunofluorescence.

Medicine

Surgical Techniques to Optimize Ovarian Reserve during Laparoscopic Cystectomy for Ovarian Endometrioma

Kathryn Saturnino^1,2,3, Osaro Obanor^1,2,3, Cynthia Arvizo⁴, Julian A. Gingold^2,3,5

¹Department of Obstetrics & Gynecology and Women's Health, Montefiore Medical Center, ²Albert Einstein College of Medicine, ³Department of OB/GYN, 1400 Pelham Pkwy S, Jacobi Medical Center, ⁴Division of Minimally Invasive Gynecologic Surgery, Department of OB/GYN, Jacobi Medical Center, ⁵Division of Reproductive Endocrinology and Infertility, Department of Obstetrics & Gynecology and Women's Health, Montefiore Medical Center

This protocol presents techniques to laparoscopically excise ovarian endometrioma, to perform adhesiolysis with sparing electrosurgical application, and to employ intraoperative chromopertubation to assess for genital tract patency. This systematic approach will facilitate optimal endometriosis management, guide concomitant adnexal surgeries, and enhance post-surgical fertility outcomes.