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NATIONAL INSTITUTE ON AGING

2 ARTICLES PUBLISHED IN JoVE

Medicine

A Rat Carotid Balloon Injury Model to Test Anti-vascular Remodeling Therapeutics

Natalia Petrasheskaya¹, Hyun-Jin Tae^1,2, Ismayil Ahmet¹, Mark I. Talan¹, Edward G. Lakatta¹, Li Lin¹

¹Laboratory of Cardiovascular Sciences, National Institute on Aging, ²Department of Biomedical Science and Research Institute for Bioscience and Biotechnology, Hallym University

The rat carotid balloon injury model described below allows researchers to evaluate drugs or therapeutics that negate injury-induced arterial hyperplasia. Detailed pre-surgical preparation, surgical procedure, and post-surgical cares of the animal are described.

Medicine

Phosphorus-31 Magnetic Resonance Spectroscopy: A Tool for Measuring In Vivo Mitochondrial Oxidative Phosphorylation Capacity in Human Skeletal Muscle

Vidhya Kumar¹, Henry Chang¹, David A. Reiter², David P. Bradley³, Martha Belury⁴, Shana E. McCormack⁵, Subha V. Raman¹

¹Davis Heart and Lung Research Institute, The Ohio State University, ²Laboratory of Clinical Investigation, National Institute on Aging, ³Division of Endocrinology, Diabetes and Metabolism, The Ohio State University, ⁴Department of Human Sciences, Human Nutrition, The Ohio State University, ⁵Division of Endocrinology and Diabetes, Department of Pediatrics, University of Pennsylvania

This work demonstrates the feasibility of an in vivo phosphorus-31 magnetic resonance spectroscopy (³¹PMRS) technique to quantify mitochondrial oxidative phosphorylation (OXPHOS) capacity in human skeletal muscle.