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Arkansas State University

3 ARTICLES PUBLISHED IN JoVE

Chemistry

Profiling the Triacylglyceride Contents in Bat Integumentary Lipids by Preparative Thin Layer Chromatography and MALDI-TOF Mass Spectrometry

Evan L. Pannkuk¹, Thomas S. Risch², Brett J. Savary³

¹Graduate Program of Environmental Science, Arkansas State University, ²Department of Biological Sciences, Arkansas State University, ³Arkansas Biosciences Institute and College of Agriculture and Technology, Arkansas State University

Mammalian integument contains solvent-extractable lipids that can provide chemical compositions characteristic of individual species. This paper presents a routine method for separating broad lipid classes isolated from integumentary tissues using thin layer chromatography and determining the triacylglyceride profile by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.

Neuroscience

Assessment of Dendritic Arborization in the Dentate Gyrus of the Hippocampal Region in Mice

Devsmita Das^1,2, Cristy Phillips³, Bill Lin¹, Fatemeh Mojabi¹, Mehmet Akif Baktir², Van Dang^1,2, Ravikumar Ponnusamy¹, Ahmad Salehi^1,2

¹VA Palo Alto Health Care System, ²Department of Psychiatry and Behavioral Sciences, Stanford University School of Medicine, ³Department of Physical Therapy, Arkansas State University

We describe two methods for visualization and quantification of dendritic arborization in the hippocampus of mouse models: real-time and extended depth of field imaging. While the former method allows sophisticated topographical tracing and quantification of the extent of branching, the latter allows speedy visualization of the dendritic tree.

Medicine

Bridging the Technology Divide in the COVID-19 Era: Using Virtual Outreach to Expose Middle and High School Students to Imaging Technology

Kevin D. Phelan¹, Mohsin Syed¹, Noor Akhter¹, Tiffany W. Huitt¹, Gregory R. Snead², Billy R. Thomas³, Karen L. Yanowitz⁴

¹Division of Clinical Anatomy, Department of Neurobiology and Developmental Sciences, College of Medicine, University of Arkansas for Medical Sciences, ²Department of Emergency Medicine, College of Medicine, University of Arkansas for Medical Sciences, ³Department of Pediatrics, College of Medicine, University of Arkansas for Medical Sciences, ⁴Department of Psychology and Counseling, Arkansas State University

This article presents an overview of how synchronous web-based virtual outreach can be used to expose 6th-12th grade students to advanced imaging technologies such as ultrasound, computerized tomography, and electroencephalography. The paper discusses the methods and equipment needed to livestream integrated educational sessions for effective student engagement in STEM.