Assessment of Glutamine as a Fuel Source for Alveolar Macrophages Exposed to Chronic Ethanol Using an Extracellular Flux BioanalyzerKathryn M. Crotty 1,2, Samantha M. Yeligar 1,2
1Department of Medicine, Division of Pulmonary, Allergy, Critical Care and Sleep Medicine, Emory University, 2Atlanta Veterans Affairs Health Care System
Alcohol misuse impairs alveolar macrophage (AM) immunity due to suppressed mitochondrial respiration and bioenergetics. We recently demonstrated that ethanol (EtOH) exposure increases glutamine dependency for mitochondrial respiration in AMs. Herein, methods are provided to determine the usage of glutamine for mitochondrial respiration in EtOH-treated AMs using an extracellular flux bioanalyzer.
