University of Texas Health Science Center at Houston - McGovern Medical School

1 ARTICLES PUBLISHED IN JoVE

Procedures for In Vitro Cultivation of <i>Treponema pallidum</i>, the Syphilis Spirochete

Procedures for In Vitro Cultivation of Treponema pallidum, the Syphilis Spirochete

Diane G. Edmondson¹, Steven J. Norris¹, Bridget D. De Lay¹

¹University of Texas Health Science Center at Houston - McGovern Medical School

This protocol describes in vitro cultivation of the syphilis pathogen Treponema pallidum subsp. pallidum in co-culture with mammalian cells. The method is scalable; it can be used to produce large quantities of T. pallidum and to generate clonal cultures.