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University of Texas Health Science Center at Houston - McGovern Medical School

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Procedures for In Vitro Cultivation of Treponema pallidum, the Syphilis Spirochete
Diane G. Edmondson 1, Steven J. Norris 1, Bridget D. De Lay 1
1University of Texas Health Science Center at Houston - McGovern Medical School

This protocol describes in vitro cultivation of the syphilis pathogen Treponema pallidum subsp. pallidum in co-culture with mammalian cells. The method is scalable; it can be used to produce large quantities of T. pallidum and to generate clonal cultures.

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