Establishment and Culture of Patient-Derived Breast OrganoidsDisha Aggarwal 1,2, Suzanne Russo 1, Payal Naik 1, Sonam Bhatia 1, David L. Spector 1,2
1Cold Spring Harbor Laboratory, Cold Spring Harbor, 2Genetics Graduate Program, Stony Brook University
A detailed protocol is provided here for establishing human breast organoids from patient-derived breast tumor resections or normal breast tissue. The protocol provides comprehensive step-by-step instructions for culturing, freezing, and thawing human patient-derived breast organoids.
