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Universidad Francisco de Vitoria

2 ARTICLES PUBLISHED IN JoVE

Biology

Stimulation of Stem Cell Niches and Tissue Regeneration in Mouse Skin by Switchable Protoporphyrin IX-Dependent Photogeneration of Reactive Oxygen Species In Situ

Jesús Espada^1,2, Elisa Carrasco³, María I. Calvo-Sánchez^1,4, Sandra Fernández-Martos¹, Juan José Montoya⁵

¹Ramón y Cajal Institute for Health Research (IRYCIS), Ramón y Cajal University Hospital, ²Centro Integrativo de Biología y Química Aplicada (CIBQA), Universidad Bernardo O'Higgins, ³Department of Molecular Biology, Faculty of Sciences, Universidad Autónoma de Madrid (UAM), ⁴Instituto de Investigaciones Biosanitarias, Facultad de Ciencias Experimentales, Universidad Francisco de Vitoria, ⁵Department of Radiology, Rehabilitation & Physiotherapy, Faculty of Medicine, Complutense University

The aim of this protocol is to induce transient in vivo production of nonlethal levels of reactive oxygen species (ROS) in mouse skin, further promoting physiological responses in the tissue.

Neuroscience

Coculture of Axotomized Rat Retinal Ganglion Neurons with Olfactory Ensheathing Glia, as an In Vitro Model of Adult Axonal Regeneration

María Portela-Lomba^1,3, Diana Simón¹, Cristina Russo², Javier Sierra¹, María Teresa Moreno-Flores³

¹Facultad de CC Experimentales, Universidad Francisco de Vitoria, ²Dept. Biomedical and Biotechnological Sciences, Section of Physiology, University of Catania, ³Dept. Anatomía, Histología y Neurociencia, Facultad de Medicina, Universidad Autónoma de Madrid

We present an in vitro model to assess olfactory ensheathing glia (OEG) neuroregenerative capacity, after neural injury. It is based on a coculture of axotomized adult retinal ganglion neurons (RGN) on OEG monolayers and subsequent study of axonal regeneration, by analyzing RGN axonal and somatodendritic markers.