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Singapore Institute of Technology

1 ARTICLES PUBLISHED IN JoVE

Genetics

Genetic Engineering of an Unconventional Yeast for Renewable Biofuel and Biochemical Production

Ai-Qun Yu^1,2, Nina Pratomo^1,2, Tee-Kheang Ng^1,2, Hua Ling^1,2, Han-Saem Cho^1,2, Susanna Su Jan Leong^1,2,3, Matthew Wook Chang^1,2

¹Department of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore, ²NUS Synthetic Biology for Clinical and Technological Innovation (SynCTI), Life Sciences Institute, National University of Singapore, ³Food Science and Chemical Engineering, Singapore Institute of Technology

We herein report methods on the molecular genetic manipulation of the Yarrowia lipolytica Po1g strain for improved gene deletion efficiency. The resulting engineered Y. lipolytica strains have potential applications in biofuel and biochemical production.