Genetic Engineering of an Unconventional Yeast for Renewable Biofuel and Biochemical ProductionAi-Qun Yu 1,2, Nina Pratomo 1,2, Tee-Kheang Ng 1,2, Hua Ling 1,2, Han-Saem Cho 1,2, Susanna Su Jan Leong 1,2,3, Matthew Wook Chang 1,2
1Department of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore, 2NUS Synthetic Biology for Clinical and Technological Innovation (SynCTI), Life Sciences Institute, National University of Singapore, 3Food Science and Chemical Engineering, Singapore Institute of Technology
We herein report methods on the molecular genetic manipulation of the Yarrowia lipolytica Po1g strain for improved gene deletion efficiency. The resulting engineered Y. lipolytica strains have potential applications in biofuel and biochemical production.
