Hi-C: A Method to Study the Three-dimensional Architecture of Genomes.Nynke L. van Berkum *1, Erez Lieberman-Aiden *2,3,4,5, Louise Williams *2, Maxim Imakaev 6, Andreas Gnirke 2, Leonid A. Mirny 3,6, Job Dekker 1, Eric S. Lander 2,7,8
1Program in Gene Function and Expression, Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, 2Broad Institute of Harvard and Massachusetts Institute of Technology, 3Division of Health Sciences and Technology, Massachusetts Institute of Technology, 4Program for Evolutionary Dynamics, Department of Organismic and Evolutionary Biology, Department of Mathematics, Harvard University , 5Department of Applied Mathematics, Harvard University , 6Department of Physics, Massachusetts Institute of Technology, 7Department of Systems Biology, Harvard Medical School, 8Department of Biology, Massachusetts Institute of Technology
The Hi-C method allows unbiased, genome-wide identification of chromatin interactions (1). Hi-C couples proximity ligation and massively parallel sequencing. The resulting data can be used to study genomic architecture at multiple scales: initial results identified features such as chromosome territories, segregation of open and closed chromatin, and chromatin structure at the megabase scale.
