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4 ARTICLES PUBLISHED IN JoVE

Bioengineering

A Droplet-Based Microfluidic Approach and Microsphere-PCR Amplification for Single-Stranded DNA Amplicons

Se Hee Lee^*1, Ho Won Lee^*2, Da Som Kim², Hyuck Gi Kwon³, Jong Hyun Lee³, Yang-Hoon Kim¹, Ok Chan Jeong^2,3, Ji-Young Ahn¹

¹College of Natural Sciences, Chungbuk National University, ²Department of Biomedical Engineering, Inje University, ³Institute of Digital Anti-Aging Healthcare, Inje University

This work provides a method for the fabrication of droplet-based microfluidic platforms and the application of polyacrylamide microspheres for microsphere-PCR amplification. The microsphere-PCR method makes it possible to obtain single-stranded DNA amplicons without separating double-stranded DNA.

Behavior

Using a Virtual Reality Walking Simulator to Investigate Pedestrian Behavior

Hyun Chae Chung¹, Soon Ho Kim², Gyoojae Choi³, Jong Won Kim⁴, Moo Young Choi², Hui Li¹

¹Department of Sport and Exercise Sciences, Kunsan National University, ²Department of Physics and Astronomy, Seoul National University, ³School of Mechanical and Automotive Engineering, Kunsan National University, ⁴Department of Healthcare Information Technology, Inje University

This protocol describes use of a walking simulator that serves as a safe and ecologically valid method to study pedestrian behavior in the presence of moving traffic.

Engineering

Microfluidic Acoustophoresis for Flowthrough Separation of Gram-Negative Bacteria using Aptamer Affinity Beads

Hong Jin Choi¹, Byung Woo Kim², SangWook Lee³, Ok Chan Jeong^1,2,4

¹Department of Digital Anti-Aging Health Care, Inje University, ²Micro device Lab Co., Ltd, ³Biohealth Products Research Center (BPRC), Inje University, ⁴Department of Biomedical Engineering, Inje University

This paper describesthe fabrication and operation of microfluidic acoustophoretic chips using the microfluidic acoustophoresis technique and aptamer-modified microbeads that can be used for fast, efficient isolation of Gram-negative bacteria from a medium.

Engineering

Pneumatically Driven Microfluidic Platform for Micro-Particle Concentration

Hong Jin Choi¹, Jong Hyun Lee², Ok Chan Jeong^1,2,3

¹Department of Digital Anti-Aging Health Care, Inje University, ²Micro device Lab Co., Ltd, ³Department of Biomedical Engineering, Inje University

The present protocol describes a pneumatic microfluidic platform that can be used for efficient microparticle concentration.