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Universidad Peruana Cayetano Heredia

2 ARTICLES PUBLISHED IN JoVE

Biology

The MODS method for diagnosis of tuberculosis and multidrug resistant tuberculosis

Mark F Brady¹, Jorge Coronel², Robert H Gilman³, David AJ Moore⁴

¹The Warren Alpert Medical School of Brown University, ²Laboratorio de Investigacion de Enfermedades Infecciosas, Universidad Peruana Cayetano Heredia, ³Department of International Health, Johns Hopkins Bloomberg School of Public Health, ⁴Wellcome Trust Centre for Clinical Tropical Medicine, Imperial College London

The microscopic-observation drug-susceptibility (MODS) assay is a low-cost, low-tech tool for high-performance detection of tuberculosis (TB) and multidrug-resistant tuberculosis (MDRTB). This video describes the MODS liquid media culture method.

Medicine

Detection and Genogrouping of Noroviruses from Children's Stools By Taqman One-step RT-PCR

Sonia Apaza¹, Susan Espetia¹, Robert H. Gilman^1,2, Sonia Montenegro³, Susana Pineda³, Fanny Herhold¹, Romeo Pomari¹, Margaret Kosek², Nancy Vu¹, Mayuko Saito^1,2,4

¹Laboratorio de Investigación y Desarrollo (LID), Universidad Peruana Cayetano Heredia, ²Bloomberg School of Public Health, Johns Hopkins University, ³Laboratorio de Diagnostico Molecular, Facultad de Medicina, University of Concepcion,Chile, ⁴University of California San Diego School of Medicine

A One-Step RT-PCR assay for detection and genogroup identification of Norovirus isolates from children’s stools, that utilizes primers and TaqMan probes specific to the open reading frame 1 (ORF1)-ORF2 junction region, the most conserved region of the Norovirus genome is described. A non-commercial, cost-effective RNA extraction method is detailed.