Universidad Peruana Cayetano Heredia

3 ARTICLES PUBLISHED IN JoVE

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Biology

The MODS method for diagnosis of tuberculosis and multidrug resistant tuberculosis
Mark F Brady 1, Jorge Coronel 2, Robert H Gilman 3, David AJ Moore 4
1The Warren Alpert Medical School of Brown University, 2Laboratorio de Investigacion de Enfermedades Infecciosas, Universidad Peruana Cayetano Heredia, 3Department of International Health, Johns Hopkins Bloomberg School of Public Health, 4Wellcome Trust Centre for Clinical Tropical Medicine, Imperial College London

The microscopic-observation drug-susceptibility (MODS) assay is a low-cost, low-tech tool for high-performance detection of tuberculosis (TB) and multidrug-resistant tuberculosis (MDRTB). This video describes the MODS liquid media culture method.

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Medicine

Detection and Genogrouping of Noroviruses from Children's Stools By Taqman One-step RT-PCR
Sonia Apaza 1, Susan Espetia 1, Robert H. Gilman 1,2, Sonia Montenegro 3, Susana Pineda 3, Fanny Herhold 1, Romeo Pomari 1, Margaret Kosek 2, Nancy Vu 1, Mayuko Saito 1,2,4
1Laboratorio de Investigación y Desarrollo (LID), Universidad Peruana Cayetano Heredia, 2Bloomberg School of Public Health, Johns Hopkins University, 3Laboratorio de Diagnostico Molecular, Facultad de Medicina, University of Concepcion,Chile, 4University of California San Diego School of Medicine

A One-Step RT-PCR assay for detection and genogroup identification of Norovirus isolates from children’s stools, that utilizes primers and TaqMan probes specific to the open reading frame 1 (ORF1)-ORF2 junction region, the most conserved region of the Norovirus genome is described. A non-commercial, cost-effective RNA extraction method is detailed.

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Bioengineering

Utilizing Whole-Cell Biosensors to Measure Ionic Mercury in Water Samples
Dahlin Zevallos-Aliaga 1, Frank Britto-Bisso 1, Nicolás A. Vaccari 1, Maarten Dequanter 2, Stijn De Graeve 3, Tom Peeters 3, Daniel G. Guerra 1
1Laboratorio de Moléculas Individuales, Laboratorios de Investigación y Desarrollo, Facultad de Ciencias e Ingeniería, Universidad Peruana Cayetano Heredia, 2Kenniscentrum AI, Erasmushogeschool Brussel, 3Open BioLab Brussels, Erasmushogeschool Brussel

This article presents protocols for utilizing two whole-cell biosensors (Mer-Blue and Mer-RFP) to detect ionic mercury. By providing detailed procedures for their operation and output analysis, the study aims to facilitate broader adoption and further development of these technologies for monitoring specific pollutants.