In Vitro Model of Human Cutaneous Hypertrophic Scarring using Macromolecular CrowdingChen Fan 1, Lay Keng Priscilla Lim 1, Zihao Wu 1, Bhavya Sharma 1, Shi Qi Gan 1,2, Kun Liang 1, Zee Upton 1,3, David Leavesley 1
1Skin Research Institute of Singapore, Agency for Science, Technology and Research (A*STAR), 2School of Chemical and Life Sciences, Singapore Polytechnic, 3Institute of Medical Biology, Agency for Science, Technology and Research (A*STAR)
This protocol describes the use of macromolecular crowding to create an in vitro human hypertrophic scar tissue model that resembles in vivo conditions. When cultivated in a crowded macromolecular environment, human skin fibroblasts exhibit phenotypes, biochemistry, physiology, and functional characteristics resembling scar tissue.
