Singapore Polytechnic

1 ARTICLES PUBLISHED IN JoVE

Bioengineering

In Vitro Model of Human Cutaneous Hypertrophic Scarring using Macromolecular Crowding

Chen Fan¹, Lay Keng Priscilla Lim¹, Zihao Wu¹, Bhavya Sharma¹, Shi Qi Gan^1,2, Kun Liang¹, Zee Upton^1,3, David Leavesley¹

¹Skin Research Institute of Singapore, Agency for Science, Technology and Research (A*STAR), ²School of Chemical and Life Sciences, Singapore Polytechnic, ³Institute of Medical Biology, Agency for Science, Technology and Research (A*STAR)

This protocol describes the use of macromolecular crowding to create an in vitro human hypertrophic scar tissue model that resembles in vivo conditions. When cultivated in a crowded macromolecular environment, human skin fibroblasts exhibit phenotypes, biochemistry, physiology, and functional characteristics resembling scar tissue.