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University of Basel, Basel, Switzerland

2 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Determining Soil-transmitted Helminth Infection Status and Physical Fitness of School-aged Children

Peiling Yap^1,2, Thomas Fürst^1,2, Ivan Müller^1,2, Susi Kriemler^1,2, Jürg Utzinger^1,2, Peter Steinmann^1,2

¹Department of Epidemiology and Public Health, Swiss Tropical and Public Health Institute, Basel, Switzerland, ²University of Basel, Basel, Switzerland

Chronic infection with soil-transmitted helminths (STHs) causes malabsorption, stunting, and wasting in the growing child. Hence, it is plausible that these infections also reduce the physical fitness of children. Here, we visualize two techniques for the diagnosis of STHs and the 20-meter shuttle run test for assessing children's physical fitness.

Environment

A Loop-mediated Isothermal Amplification (LAMP) Assay for Rapid Identification of Bemisia tabaci

Simon Blaser^1,2,3, Hanspeter Diem⁴, Andreas von Felten⁴, Morgan Gueuning¹, Michael Andreou⁵, Neil Boonham^6,7, Jennifer Tomlinson⁶, Pie Müller^2,3, Jürg Utzinger^2,3, Beatrice Frey¹, Jürg E. Frey¹, Andreas Bühlmann⁸

¹Department of Method Development and Analytics, Agroscope, ²Swiss Tropical and Public Health Institute, ³University of Basel, ⁴Swiss Federal Plant Protection Service, Federal Office for Agriculture, ⁵OptiGene Limited, ⁶Fera Science Limited, ⁷School of Natural and Environmental Sciences, Newcastle University, ⁸Department of Plants and Plant Products, Agroscope

This paper reports the protocol for a rapid identification assay for Bemisia tabaci based on loop-mediated isothermal amplification (LAMP) technology. The protocol requires minimal laboratory training and can, therefore, be implemented on-site at points of entry for plant imports such as seaports and airports.