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Boyce Thompson Institute for Plant Research

3 ARTICLES PUBLISHED IN JoVE

Biology

Virus-induced Gene Silencing (VIGS) in Nicotiana benthamiana and Tomato

Andrá C. Velásquez^1,2, Suma Chakravarthy², Gregory B. Martin^1,2

¹Plant Pathology and Plant-Microbe Biology, Cornell University, ²Boyce Thompson Institute for Plant Research

Description of a virus-induced gene silencing (VIGS) method for knock-down of gene expression in Nicotiana benthamiana and tomato.

Immunology and Infection

Assay for Pathogen-Associated Molecular Pattern (PAMP)-Triggered Immunity (PTI) in Plants

Suma Chakravarthy¹, André C. Velásquez^1,2, Gregory B. Martin^1,2

¹Boyce Thompson Institute for Plant Research, ²Plant Pathology and Plant-Microbe Biology, Cornell University

A cell death-based assay for PTI in Nicotiana benthamiana plants is described.

Biology

High-throughput CRISPR Vector Construction and Characterization of DNA Modifications by Generation of Tomato Hairy Roots

Thomas B. Jacobs¹, Gregory B. Martin^1,2

¹Boyce Thompson Institute for Plant Research, ²Section of Plant Pathology & Plant-Microbe Biology, School of Integrative Plant Science, Cornell University

Using DNA assembly, multiple CRISPR vectors can be constructed in parallel in a single cloning reaction, making the construction of large numbers of CRISPR vectors a simple task. Tomato hairy roots are an excellent model system to validate CRISPR vectors and generate mutant materials.