Boyce Thompson Institute for Plant Research
3 ARTICLES PUBLISHED IN JoVE
Biology
Virus-induced Gene Silencing (VIGS) in Nicotiana benthamiana and Tomato
Andrá C. Velásquez 1,2, Suma Chakravarthy 2, Gregory B. Martin 1,2
1Plant Pathology and Plant-Microbe Biology, Cornell University, 2Boyce Thompson Institute for Plant Research
Description of a virus-induced gene silencing (VIGS) method for knock-down of gene expression in Nicotiana benthamiana and tomato.
Immunology and Infection
Assay for Pathogen-Associated Molecular Pattern (PAMP)-Triggered Immunity (PTI) in Plants
Suma Chakravarthy 1, André C. Velásquez 1,2, Gregory B. Martin 1,2
1Boyce Thompson Institute for Plant Research, 2Plant Pathology and Plant-Microbe Biology, Cornell University
A cell death-based assay for PTI in Nicotiana benthamiana plants is described.
Biology
High-throughput CRISPR Vector Construction and Characterization of DNA Modifications by Generation of Tomato Hairy Roots
Thomas B. Jacobs 1, Gregory B. Martin 1,2
1Boyce Thompson Institute for Plant Research, 2Section of Plant Pathology & Plant-Microbe Biology, School of Integrative Plant Science, Cornell University
Using DNA assembly, multiple CRISPR vectors can be constructed in parallel in a single cloning reaction, making the construction of large numbers of CRISPR vectors a simple task. Tomato hairy roots are an excellent model system to validate CRISPR vectors and generate mutant materials.
