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University of Victoria-Genome BC Proteomics Centre

3 ARTICLES PUBLISHED IN JoVE

Biology

Dithranol as a Matrix for Matrix Assisted Laser Desorption/Ionization Imaging on a Fourier Transform Ion Cyclotron Resonance Mass Spectrometer

Cuong H. Le^1,2, Jun Han¹, Christoph H. Borchers^1,2

¹University of Victoria-Genome BC Proteomics Centre, University of Victoria, ²Department of Biochemistry and Microbiology, University of Victoria

Dithranol (DT; 1,8-dihydroxy-9,10-dihydroanthracen-9-one) has previously been reported as a MALDI matrix for tissue imaging of small molecules; protocols for the use of DT for the MALDI imaging of endogenous lipids on the surface of tissue sections by positive-ion MALDI-MS on an ultrahigh-resolution quadrupole-FTICR instrument are provided here.

Chemistry

Peptide and Protein Quantification Using Automated Immuno-MALDI (iMALDI)

Huiyan Li¹, Robert Popp¹, Bjorn Frohlich¹, Michael X. Chen², Christoph H. Borchers^1,3,4,5

¹University of Victoria-Genome BC Proteomics Centre, ²Jewish General Hospital, McGill University, ³Dept of Biochemistry and Microbiology, University of Victoria, ⁴Proteomics Centre, Segal Cancer Centre, Lady Davis Institute, Jewish General Hospital, McGill University, ⁵Gerald Bronfman Department of Oncology, Jewish General Hospital

A protocol for the protein quantification in complex biological fluids using automated immuno-MALDI (iMALDI) technology is presented.

Bioengineering

Snap Chip for Cross-reactivity-free and Spotter-free Multiplexed Sandwich Immunoassays

Huiyan Li^1,2, Sebastien Bergeron³, Heidi Larkin^1,2,3, David Juncker^1,2

¹McGill University and Génome Québec Innovation Centre, ²Biomedical Engineering Department, McGill University, ³Parallex BioAssays Inc.

We demonstrate a snap chip technology for performing cross-reactivity-free multiplexed sandwich immunoassays by simply snapping two slides. A snap apparatus is used for reliably transferring reagents from microarray-to-microarray. The snap chip can be used for any biochemical reactions requiring colocalization of different reagents without cross-contamination.