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Rigshospitalet

3 ARTICLES PUBLISHED IN JoVE

Neuroscience

In Vivo Three-Dimensional Two-Photon Microscopy to Study Conducted Vascular Responses by Local ATP Ejection Using a Glass Micro-Pipette

Changsi Cai¹, Stefan A. Zambach¹, Jonas C. Fordsmann¹, Micael Lønstrup¹, Kirsten J. Thomsen¹, Aske G. K. Jensen¹, Martin Lauritzen^1,2

¹Department of Neuroscience, Faculty of Medicine and Health Science, University of Copenhagen, ²Department of Clinical Neurophysiology, Rigshospitalet

We present an optimized local ejection procedure using a glass micro-pipette and a fast two-photon hyperstack imaging method, which allows precise measurement of capillary diameter changes and investigation of its regulation in three dimensions.

Immunology and Infection

Measuring Naturally Acquired Phagocytosis-Inducing Antibodies to Plasmodium falciparum Parasites by a Flow Cytometry-Based Assay

Maria del Pilar Quintana¹, Nsoh Godwin Anabire^1,2,3, Lars Hviid^1,4

¹Centre for Medical Parasitology, Department of Immunology and Microbiology, Faculty of Health and Medical Sciences, University of Copenhagen, ²West African Centre for Cell Biology of Infectious Pathogens, Department of Biochemistry, Cell and Molecular Biology, University of Ghana, ³Department of Immunology, Noguchi Memorial Institute for Medical Research, ⁴Centre for Medical Parasitology, Department of Infectious Diseases, Rigshospitalet

The overall goal of this protocol is to provide instruction on how to measure the capacity of antibodies present in sera or plasma of individuals, naturally exposed to Plasmodium falciparum infection, to opsonize and induce phagocytosis of the parasite-infected erythrocytes (IEs).

Neuroscience

Pre-Chiasmatic, Single Injection of Autologous Blood to Induce Experimental Subarachnoid Hemorrhage in a Rat Model

Jesper Peter Bömers^1,2, Sara Ellinor Johansson², Lars Edvinsson^2,4, Tiit Illimar Mathiesen^1,3,5, Kristian Agmund Haanes²

¹Department of Neurosurgery, Rigshospitalet, ²Department of Clinical Experimental Research, Glostrup Research Institute, Rigshospitalet, ³Department of Clinical Medicine, University of Copenhagen, ⁴Department of Clinical Sciences, Division of Experimental Vascular Research, Lund University, ⁵Department of Clinical Neuroscience, Karolinska Institutet

Subarachnoid hemorrhage continues to carry a high burden of mortality and morbidity in man. To facilitate further research into the condition and its pathophysiology, a pre-chiasmatic, single injection model is presented.