Automated Quantification of Hematopoietic Cell – Stromal Cell Interactions in Histological Images of Undecalcified BoneSandra Zehentmeier 1, Zoltan Cseresnyes 2,3, Juan Escribano Navarro 4, Raluca A. Niesner 2, Anja E. Hauser 1,5
1Immunodynamics, German Rheumatism Research Center, a Leibniz Institute, 2Biophysical Analytics, German Rheumatism Research Center, a Leibniz Institute, 3Max-Delbrück Center for Molecular Medicine, 4Wimasis GmbH, 5Immunodynamics and Intravital Imaging, Charité - University of Medicine
A strategy to quantitatively analyze histological data in the bone marrow is presented. Confocal microscopy of fluorescently labeled cells in tissue sections results in 2-dimensional images, which are automatically analyzed. Co-localization analyses of different cell types are compared to data from simulated images, giving quantitative information about cellular interactions.
