Using the GAL4-UAS System for Functional Genetics in Anopheles gambiaeBeth Crawford Poulton 1, Fraser Colman 1, Amalia Anthousi 1,2,3, Linda Grigoraki 1, Adriana Adolfi 1, Amy Lynd 1, Gareth John Lycett 1
1Department of Vector Biology, Liverpool School of Tropical Medicine, 2IMBB FORTH, 3Department of Biology, University of Crete
The bipartite GAL4-UAS system is a versatile tool for modification of gene expression in a controlled spatiotemporal manner which permits functional genetic analysis in Anopheles gambiae. The procedures described for using this system are a semi-standardized cloning strategy, sexing and screening of pupae for fluorescent protein markers and embryo fixation.
