Laser Microdissection for Species-Agnostic Single-Tissue ApplicationsAlyssa Woronik 1,2, Karin Kiontke 1, Raya S. Jallad 1, R. Antonio Herrera 3, David H. A. Fitch 1
1Center for Developmental Genetics, New York University, 2Sacred Heart University, 3Baylor School
A protocol is described that uses laser microdissection to isolate individual nematode tissues for RNA-sequencing. The protocol does not require species-specific genetic toolkits, allowing gene expression profiles to be compared between different species at the level of single-tissue samples.
