Use of Electron Paramagnetic Resonance in Biological Samples at Ambient Temperature and 77 KHanan B. Elajaili 1, Laura Hernandez-Lagunas 1, Kalina Ranguelova 2, Sergey Dikalov 3, Eva Nozik-Grayck 1
1Cardiovascular Pulmonary Research Laboratories and Pediatric Critical Care Medicine, Department of Pediatrics, University of Colorado Anschutz Medical Campus, 2Bruker BioSpin Corp, 3Department of Medicine, Division of Clinical Pharmacology, Vanderbilt University Medical Center
Electron paramagnetic resonance (EPR) spectroscopy is an unambiguous method to measure free radicals. The use of selective spin probes allows for detection of free radicals in different cellular compartments. We present a practical, efficient method to collect biological samples that facilitate treating, storing, and transferring samples for EPR measurements.
