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Chuo University

2 ARTICLES PUBLISHED IN JoVE

Environment

Measurement of the Potential Rates of Dissimilatory Nitrate Reduction to Ammonium Based on ¹⁴NH₄⁺/¹⁵NH₄⁺ Analyses via Sequential Conversion to N₂O

Megumi Kuroiwa¹, Keitaro Fukushima^2,3, Kazuma Hashimoto², Yukiko Senga⁴, Tsubasa Sato⁴, Chie Katsuyama⁵, Yuichi Suwa¹

¹Department of Biological Sciences, Faculty of Science and Engineering, Chuo University, ²Faculty & Graduate School of Urban Environmental Sciences, Tokyo Metropolitan University, ³Center for Ecological Research, Kyoto University, ⁴Department of Chemistry, Faculty of Science, Toho University, ⁵Graduate School of Integrated Arts and Sciences, Hiroshima University

A series of methods to determine the potential DNRA rate based on ¹⁴NH₄⁺/¹⁵NH₄⁺ analyses is provided in detail. NH₄⁺ is converted into N₂O via several steps and analyzed using quadrupole gas chromatography–mass spectrometry.

Medicine

Sarcomere Shortening of Pluripotent Stem Cell-Derived Cardiomyocytes using Fluorescent-Tagged Sarcomere Proteins.

Razan E. Ahmed^*1, Nawin Chanthra^*1, Tatsuya Anzai^1,2, Keiichiro Koiwai^3,4, Tomoki Murakami⁴, Hiroaki Suzuki⁴, Yutaka Hanazono¹, Hideki Uosaki¹

¹Division of Regenerative Medicine, Center for Molecular Medicine, Jichi Medical University, ²Department of Pediatrics, Jichi Medical University, ³Institute of Global Innovation Research, Tokyo University of Agriculture and Technology, ⁴Department of Precision Mechanics, Faculty of Science and Engineering, Chuo University

This method can be used to examine sarcomere shortening using pluripotent stem cell-derived cardiomyocytes with fluorescent-tagged sarcomere proteins.