Chuo University
2 ARTICLES PUBLISHED IN JoVE
Environment
Measurement of the Potential Rates of Dissimilatory Nitrate Reduction to Ammonium Based on 14NH4+/15NH4+ Analyses via Sequential Conversion to N2O
Megumi Kuroiwa 1, Keitaro Fukushima 2,3, Kazuma Hashimoto 2, Yukiko Senga 4, Tsubasa Sato 4, Chie Katsuyama 5, Yuichi Suwa 1
1Department of Biological Sciences, Faculty of Science and Engineering, Chuo University, 2Faculty & Graduate School of Urban Environmental Sciences, Tokyo Metropolitan University, 3Center for Ecological Research, Kyoto University, 4Department of Chemistry, Faculty of Science, Toho University, 5Graduate School of Integrated Arts and Sciences, Hiroshima University
A series of methods to determine the potential DNRA rate based on 14NH4+/15NH4+ analyses is provided in detail. NH4+ is converted into N2O via several steps and analyzed using quadrupole gas chromatography–mass spectrometry.
Medicine
Sarcomere Shortening of Pluripotent Stem Cell-Derived Cardiomyocytes using Fluorescent-Tagged Sarcomere Proteins.
Razan E. Ahmed *1, Nawin Chanthra *1, Tatsuya Anzai 1,2, Keiichiro Koiwai 3,4, Tomoki Murakami 4, Hiroaki Suzuki 4, Yutaka Hanazono 1, Hideki Uosaki 1
1Division of Regenerative Medicine, Center for Molecular Medicine, Jichi Medical University, 2Department of Pediatrics, Jichi Medical University, 3Institute of Global Innovation Research, Tokyo University of Agriculture and Technology, 4Department of Precision Mechanics, Faculty of Science and Engineering, Chuo University
This method can be used to examine sarcomere shortening using pluripotent stem cell-derived cardiomyocytes with fluorescent-tagged sarcomere proteins.
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