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International Institute of Molecular and Cell Biology in Warsaw

2 ARTICLES PUBLISHED IN JoVE

Biology

A Protocol for Laboratory Housing of Turquoise Killifish (Nothobranchius furzeri)

Joanna Dodzian^1,3, Sam Kean¹, Jens Seidel¹, Dario Riccardo Valenzano^1,2

¹Max Planck Institute for the Biology of Ageing, ²CECAD, University of Cologne, ³International Institute of Molecular and Cell Biology in Warsaw

Laboratory housing of turquoise killifish can be scaled up to house and efficiently raise thousands of individual fish in a centralized water filtration system, employing the same infrastructure used for standard zebrafish facilities. Here we detail a list of standardized procedures that allow efficient killifish maintenance.

Biochemistry

High-Throughput Image-Based Quantification of Mitochondrial DNA Synthesis and Distribution

Lukasz S. Borowski^1,2, Karolina Kasztelan^2,3, Jolanta Czerwinska-Kostrzewska^1,2, Roman J. Szczesny²

¹Faculty of Biology, Institute of Genetics and Biotechnology, University of Warsaw, ²Institute of Biochemistry and Biophysics, Polish Academy of Sciences, ³International Institute of Molecular and Cell Biology in Warsaw

A procedure for studying the dynamics of mitochondrial DNA (mtDNA) metabolism in cells using a multi-well plate format and automated immunofluorescence imaging to detect and quantify mtDNA synthesis and distribution is described. This can be further used to investigate the effects of various inhibitors, cellular stresses, and gene silencing on mtDNA metabolism.