International Institute of Molecular and Cell Biology in Warsaw
2 ARTICLES PUBLISHED IN JoVE
Biology
A Protocol for Laboratory Housing of Turquoise Killifish (Nothobranchius furzeri)
Joanna Dodzian 1,3, Sam Kean 1, Jens Seidel 1, Dario Riccardo Valenzano 1,2
1Max Planck Institute for the Biology of Ageing, 2CECAD, University of Cologne, 3International Institute of Molecular and Cell Biology in Warsaw
Laboratory housing of turquoise killifish can be scaled up to house and efficiently raise thousands of individual fish in a centralized water filtration system, employing the same infrastructure used for standard zebrafish facilities. Here we detail a list of standardized procedures that allow efficient killifish maintenance.
Biochemistry
High-Throughput Image-Based Quantification of Mitochondrial DNA Synthesis and Distribution
Lukasz S. Borowski 1,2, Karolina Kasztelan 2,3, Jolanta Czerwinska-Kostrzewska 1,2, Roman J. Szczesny 2
1Faculty of Biology, Institute of Genetics and Biotechnology, University of Warsaw, 2Institute of Biochemistry and Biophysics, Polish Academy of Sciences, 3International Institute of Molecular and Cell Biology in Warsaw
A procedure for studying the dynamics of mitochondrial DNA (mtDNA) metabolism in cells using a multi-well plate format and automated immunofluorescence imaging to detect and quantify mtDNA synthesis and distribution is described. This can be further used to investigate the effects of various inhibitors, cellular stresses, and gene silencing on mtDNA metabolism.
关于 JoVE
版权所属 © 2024 MyJoVE 公司版权所有，本公司不涉及任何医疗业务和医疗服务。