Single Cell Collection of Trophoblast Cells in Peri-implantation Stage Human EmbryosDeirdre M. Logsdon 1, Rebecca A. Kile 1, William B. Schoolcraft 1, Rebecca L. Krisher 1, Ye Yuan 1
1Colorado Center for Reproductive Medicine
Here, we describe a method for warming vitrified human blastocysts, culturing them through the implantation period in vitro, digesting them into single cells and collecting early trophoblast cells for further investigation.
