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Colorado Center for Reproductive Medicine

1 ARTICLES PUBLISHED IN JoVE

Biology

Single Cell Collection of Trophoblast Cells in Peri-implantation Stage Human Embryos

Deirdre M. Logsdon¹, Rebecca A. Kile¹, William B. Schoolcraft¹, Rebecca L. Krisher¹, Ye Yuan¹

¹Colorado Center for Reproductive Medicine

Here, we describe a method for warming vitrified human blastocysts, culturing them through the implantation period in vitro, digesting them into single cells and collecting early trophoblast cells for further investigation.