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TVHS VA Medical Center

2 ARTICLES PUBLISHED IN JoVE

Biology

Development of a Cell Co-Culture Model to Mimic Cardiac Ischemia/Reperfusion In Vitro

Zhu Li¹, Matthew J. W. Hampton¹, Matthew B. Barajas¹, Matthias L. Riess^1,2,3

¹Department of Anesthesiology, Vanderbilt University Medical Center, ²Anesthesiology, TVHS VA Medical Center, ³Department of Pharmacology, Vanderbilt University

Spatial distance is a key parameter in assessing hypoxia/reoxygenation injury in a co-culture model of separate endothelial and cardiomyocyte cell layers, suggesting, for the first time, that optimizing the co-culture spatial environment is necessary to provide a favorable in vitro model for testing the role of endothelial cells in cardiomyocyte protection.

Current and Novel Experimental Methods in Ischemia/Reperfusion Research: From Cutting-Edge Models to Innovative Therapies

Matthew B. Barajas^1,2, Matthias L. Riess^1,2,3

¹Department of Anesthesiology, Vanderbilt University Medical Center, ²Department of Anesthesiology, TVHS VA Medical Center, ³Department of Pharmacology, Vanderbilt University

Current and Novel Experimental Methods in Ischemia/Reperfusion Research: From Cutting-Edge Models to Innovative Therapies