Microarray-based Identification of Individual HERV Loci Expression: Application to Biomarker Discovery in Prostate CancerPhilippe Pérot 1,2, Valérie Cheynet 1,2, Myriam Decaussin-Petrucci 1,3,4, Guy Oriol 1,2, Nathalie Mugnier 5, Claire Rodriguez-Lafrasse 1,4,6, Alain Ruffion 1,4,7, François Mallet 1,2
1Joint Unit Hospices de Lyon-bioMérieux, 2Medical Diagnostic Discovery Department, BioMérieux, 3Department of Pathology and Cytology, Centre Hospitalier Lyon Sud, Hospices Civils de Lyon, 4Medical Faculty, Lyon 1 University, 5Data and Knowledge Laboratory, BioMérieux, 6Department of Biochemistry and Molecular Biology, Centre Hospitalier Lyon Sud, Hospices Civils de Lyon, 7Department of Urology, Centre Hospitalier Lyon Sud, Hospices Civils de Lyon
Human endogenous retroviruses (HERV), which occupy 8% of the human genome, retain scarce coding capacities but a hundred thousand long terminal repeats (LTRs). A custom Affymetrix microarray was designed to identify individual HERV locus expression and was used on prostate cancer tissues as a proof of concept for future clinical studies.
