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Universidade da Beira Interior

2 ARTICLES PUBLISHED IN JoVE

Biology

Isolation of Human Umbilical Arterial Smooth Muscle Cells (HUASMC)

Maximiano P. Ribeiro¹, Ricardo Relvas¹, Samuel Chiquita¹, Ilídio J. Correia¹

¹Centro de Investigação em Ciências da Saúde, Faculdade de Ciências da Saúde, Universidade da Beira Interior

The umbilical cords are used to isolate smooth muscle cells by different ways. In this work we used the enzymatic treatment to isolated smooth muscle cells.

Neuroscience

A Cell Culture Model for Studying the Role of Neuron-Glia Interactions in Ischemia

Genilso Gava-Junior^*1, Cláudio Roque^*1, Julieta Mendes-Oliveira¹, Ana C Bernardino¹, Inês Serrenho¹, Joel P Pires¹, Graça Baltazar^1,2

¹Centro de Investigação em Ciências da Saúde (CICS-UBI), Universidade da Beira Interior, ²Faculdade de Ciências da Saúde, Universidade da Beira Interior

Here, we present a simple approach using specific culture media that allows the establishment of neuron- and astrocyte-enriched cultures, or neuron-glia cultures from the embryonic cortex, with high yield and reproducibility.