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Paracelsus Medical University

2 ARTICLES PUBLISHED IN JoVE

JoVE Core

Basophil Activation Test for Investigation of IgE-Mediated Mechanisms in Drug Hypersensitivity

Markus Steiner¹, Andrea Harrer², Roland Lang³, Michael Schneider⁴, Fátima Ferreira⁵, Thomas Hawranek³, Martin Himly¹

¹Department of Molecular Biology, University of Salzburg, ²Department of Neurology, Paracelsus Medical University, ³Department of Dermatology, Paracelsus Medical University, ⁴Bühlmann Laboratories, ⁵Christian Doppler Laboratory for Allergy Diagnosis and Therapy, University of Salzburg

Basophil activation test is a potent tool for the detection of IgE-dependent allergies in vitro. Here, an optimized protocol for basophil activation test is used to investigate drug hypersensitivity. A method for the efficient production of covalent drug-protein conjugates and their physicochemical characterization is described.

Biology

Cystometric and External Urethral Sphincter Measurements in Awake Rats with Implanted Catheter and Electrodes Allowing for Repeated Measurements

Elena E. Foditsch^1,2, Karin Roider^1,2, Andrea M. Sartori^3,4,5, Thomas M. Kessler⁵, Sabik Raj Kayastha⁶, Ludwig Aigner², Marc P. Schneider⁷

¹Department of Urology, Spinal Cord Injury and Tissue Regeneration Center Salzburg, Paracelsus Medical University, ²Institute of Molecular Regenerative Medicine, Spinal Cord Injury and Tissue Regeneration Center Salzburg, Paracelsus Medical University, ³Brain Research Institute, University of Zürich, ⁴Department of Health Sciences and Technology, Swiss Federal Institute of Technology Zürich, ⁵Neuro-Urology, Spinal Cord Injury Center & Research, University of Zürich, Balgrist University Hospital, ⁶Department of Orthopaedics and Traumatology, Dhulikhel Hospital, Kathmandu University Hospital, ⁷Department of Urology, Inselspital, Bern University Hospital

This protocol first describes the surgical procedure of the permanent implantation of a urinary bladder catheter combined with external urethral sphincter electrodes, and second, the measurement of the function of the urinary bladder and external urethral sphincter in implanted awake animals.