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Paracelsus Medical University

2 ARTICLES PUBLISHED IN JoVE

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JoVE Core

Basophil Activation Test for Investigation of IgE-Mediated Mechanisms in Drug Hypersensitivity
Markus Steiner 1, Andrea Harrer 2, Roland Lang 3, Michael Schneider 4, Fátima Ferreira 5, Thomas Hawranek 3, Martin Himly 1
1Department of Molecular Biology, University of Salzburg, 2Department of Neurology, Paracelsus Medical University, 3Department of Dermatology, Paracelsus Medical University, 4Bühlmann Laboratories, 5Christian Doppler Laboratory for Allergy Diagnosis and Therapy, University of Salzburg

Basophil activation test is a potent tool for the detection of IgE-dependent allergies in vitro. Here, an optimized protocol for basophil activation test is used to investigate drug hypersensitivity. A method for the efficient production of covalent drug-protein conjugates and their physicochemical characterization is described.

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Biology

Cystometric and External Urethral Sphincter Measurements in Awake Rats with Implanted Catheter and Electrodes Allowing for Repeated Measurements
Elena E. Foditsch 1,2, Karin Roider 1,2, Andrea M. Sartori 3,4,5, Thomas M. Kessler 5, Sabik Raj Kayastha 6, Ludwig Aigner 2, Marc P. Schneider 7
1Department of Urology, Spinal Cord Injury and Tissue Regeneration Center Salzburg, Paracelsus Medical University, 2Institute of Molecular Regenerative Medicine, Spinal Cord Injury and Tissue Regeneration Center Salzburg, Paracelsus Medical University, 3Brain Research Institute, University of Zürich, 4Department of Health Sciences and Technology, Swiss Federal Institute of Technology Zürich, 5Neuro-Urology, Spinal Cord Injury Center & Research, University of Zürich, Balgrist University Hospital, 6Department of Orthopaedics and Traumatology, Dhulikhel Hospital, Kathmandu University Hospital, 7Department of Urology, Inselspital, Bern University Hospital

This protocol first describes the surgical procedure of the permanent implantation of a urinary bladder catheter combined with external urethral sphincter electrodes, and second, the measurement of the function of the urinary bladder and external urethral sphincter in implanted awake animals.

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