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Sanford-Burnham Medical Research Institute

5 ARTICLES PUBLISHED IN JoVE

Neuroscience

Efficient Derivation of Human Neuronal Progenitors and Neurons from Pluripotent Human Embryonic Stem Cells with Small Molecule Induction

Xuejun H. Parsons^1,2, Yang D. Teng^3,4, James F. Parsons^1,2, Evan Y. Snyder^1,2,5, David B. Smotrich^1,2,6, Dennis A. Moore^1,2

¹San Diego Regenerative Medicine Institute, ²Xcelthera, ³Department of Neurosurgery, Harvard Medical School, ⁴Division of SCI Research, VA Boston Healthcare System, ⁵Program in Stem Cell & Regenerative Biology, Sanford-Burnham Medical Research Institute, ⁶La Jolla IVF

We have established a protocol for induction of neuroblasts direct from pluripotent human embryonic stem cells maintained under defined conditions with small molecules, which enables derivation of a large supply of human neuronal progenitors and neuronal cell types in the developing CNS for neural repair.

Biology

Efficient Derivation of Human Cardiac Precursors and Cardiomyocytes from Pluripotent Human Embryonic Stem Cells with Small Molecule Induction

Xuejun H. Parsons^1,2, Yang D. Teng^3,4, James F. Parsons^1,2, Evan Y. Snyder^1,2,5, David B. Smotrich^1,2,6, Dennis A. Moore^1,2

We have established a protocol for induction of cardioblasts direct from pluripotent human embryonic stem cells maintained under defined conditions with small molecules, which enables derivation of a large supply of human cardiac progenitors and functional cardiomyocytes for cardiovascular repair.

Medicine

Sampling Human Indigenous Saliva Peptidome Using a Lollipop-Like Ultrafiltration Probe: Simplify and Enhance Peptide Detection for Clinical Mass Spectrometry

Wenhong Zhu¹, Richard L. Gallo^2,3, Chun-Ming Huang^2,3,4

¹Sanford-Burnham Medical Research Institute, ²Division of Dermatology, University of California, San Diego , ³VA San Diego Healthcare Center, ⁴Moores Cancer Center, University of California, San Diego

Considering saliva sampling for future clinical application, a lollipop-like ultrafiltration (LLUF) probe was fabricated to fit in the human oral cavity. Direct analysis of undigested saliva by NanoLC-LTQ mass spectrometry demonstrated the ability of LLUF probes to remove large proteins and high abundance proteins, and make low-abundant peptides more detectable.

Developmental Biology

Generation of Induced Pluripotent Stem Cells from Frozen Buffy Coats using Non-integrating Episomal Plasmids

Viviana Meraviglia^*1, Alessandra Zanon^*1, Alexandros A. Lavdas¹, Christine Schwienbacher¹, Rosamaria Silipigni², Marina Di Segni², Huei-Sheng Vincent Chen³, Peter P. Pramstaller¹, Andrew A. Hicks¹, Alessandra Rossini¹

¹Center for Biomedicine, European Academy Bozen/Bolzano (EURAC), ²Laboratory of Medical Genetics, Fondazione IRCCS Ca´ Granda, Ospedale Maggiore Policlinico, ³Del E. Webb Center for Neuroscience, Aging & Stem Cell Research, Sanford-Burnham Medical Research Institute

Induced pluripotent stem cells (iPSCs) represent a source of patient-specific tissues for clinical applications and basic research. Here, we present a detailed protocol to reprogram human peripheral blood mononuclear cells (PBMNCs) obtained from frozen buffy coats into viral-free iPSCs using non-integrating episomal plasmids.

Developmental Biology

Generation of 3D Whole Lung Organoids from Induced Pluripotent Stem Cells for Modeling Lung Developmental Biology and Disease

Sandra L. Leibel^1,2,3, Rachael N. McVicar^2,3, Alicia M. Winquist^2,3, Evan Y. Snyder^1,2,3

¹Department of Pediatrics, University of California, San Diego School of Medicine, ²Sanford Consortium for Regenerative Medicine, ³Sanford Burnham Prebys Medical Discovery Institute

The article describes step wise directed differentiation of induced pluripotent stem cells to three-dimensional whole lung organoids containing both proximal and distal epithelial lung cells along with mesenchyme.