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Erasmus MC

8 ARTICLES PUBLISHED IN JoVE

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Neuroscience

Three Dimensional Vestibular Ocular Reflex Testing Using a Six Degrees of Freedom Motion Platform
Joyce Dits 1, Mark M.J. Houben 2, Johannes van der Steen 1
1Department of Neuroscience, Erasmus MC, 2TNO Human Factors

A method is described to measure three-dimensional vestibulo ocular reflexes (3D VOR) in humans using a six degrees of freedom (6DF) motion simulator. The gain and misalignment of the 3D angular VOR provide a direct measure of the quality of vestibular function. Representative data on healthy subjects are provided

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Bioengineering

In Vivo Quantitative Assessment of Myocardial Structure, Function, Perfusion and Viability Using Cardiac Micro-computed Tomography
Elza van Deel 1,2, Yanto Ridwan 1, J. Nicole van Vliet 1, Sasha Belenkov 3, Jeroen Essers 1,4,5
1Department of Genetics, Erasmus MC, Rotterdam, 2Department of Experimental Cardiology, Erasmus MC, Rotterdam, 3PerkinElmer, Inc., 4Department of Vascular Surgery, Erasmus MC, Rotterdam, 5Department of Radiation Oncology, Erasmus MC, Rotterdam

This method outlines the use of Quantum Micro-Computed Tomography (MicroCT) to assess cardiac morphology, function, perfusion, metabolism and viability with iodinated contrast agent in mice with experimentally-induced myocardial ischemia. The technique can be applied for non-destructive high-throughput longitudinal in vivo imaging of various animal models of human heart disease.

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Medicine

Surgical Placement of Catheters for Long-term Cardiovascular Exercise Testing in Swine
Daphne P M De Wijs-Meijler *1, Kelly Stam *2, Richard W B van Duin 1, Annemarie Verzijl 2, Irwin K Reiss 3, Dirk J Duncker 2, Daphne Merkus 2
1Experimental Cardiology and Neonatology, Erasmus MC, 2Experimental Cardiology, Erasmus MC, 3Neonatology, Erasmus MC

Here we present a protocol to assess cardiopulmonary function in awake swine, at rest and during graded treadmill exercise. Chronic instrumentation allows for repeated hemodynamic measurements uninfluenced by cardiodepressive anesthetic agents.

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Behavior

A Method to Quantify Visual Information Processing in Children Using Eye Tracking
Marlou J.G. Kooiker 1, Johan J.M. Pel 1, Sanny P. van der Steen-Kant 2, Johannes van der Steen 1,2
1Department of Neuroscience, Erasmus MC, 2KEI, Royal Dutch Visio

A method is described to quantify the quality of visual information processing based on reflexive eye movements in response to specific visual modalities. Reaction times and fixation output parameters are used to characterize visual performance in children with and without visual impairments from 6 months of age.

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Medicine

Intravital Microscopy of Tumor-associated Vasculature Using Advanced Dorsal Skinfold Window Chambers on Transgenic Fluorescent Mice
Ann L.B. Seynhaeve 1, Timo L.M. ten Hagen 1
1Laboratory Experimental Surgical Oncology, Section Surgical Oncology, Department of Surgery, Erasmus MC

This protocol describes the intravital imaging of transgenic mice expressing cell-specific fluorescent markers. Intravital imaging provides a non-invasive method for high-resolution observations in living animals using implantable windows through which the microscopic visualization of processes is possible. This method is especially useful to study longitudinal processes.

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Medicine

Using In Vitro Live-cell Imaging to Explore Chemotherapeutics Delivered by Lipid-based Nanoparticles
Ann L.B. Seynhaeve 1, Timo L.M. ten Hagen 1
1Laboratory Experimental Surgical Oncology, Section Surgical Oncology, Department of Surgery, Erasmus MC

Live-cell imaging is a powerful tool to visualize dynamic processes. The examination of fixed cells provides only static pictures, which can lead to misinterpretation and confusion about the process. This work presents a method to study uptake, drug release, and intracellular localization of liposomal nanoparticles in living cells.

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Immunology and Infection

A Multimodal Imaging Approach Based on Micro-CT and Fluorescence Molecular Tomography for Longitudinal Assessment of Bleomycin-Induced Lung Fibrosis in Mice
Francesca Ruscitti 1, Francesca Ravanetti 2, Gaetano Donofrio 2, Yanto Ridwan 3, Paula van Heijningen 3, Jeroen Essers 4, Gino Villetti 1, Antonio Cacchioli 2, Wim Vos 5, Franco Fabio Stellari 1
1Corporate Preclinical R&D, Chiesi Farmaceutici S.p.A., 2Department of Veterinary Science, University of Parma, 3Department of Molecular Genetics, Erasmus MC, 4Department of Molecular Genetics, Vascular Surgery, Radiation Oncology, Erasmus MC, 5Fluidda NV

We describe a non-invasive multimodal imaging approach based on Micro-CT and fluorescence molecular tomography for longitudinal assessment of the mouse lung fibrosis model induced by double intratracheal instillation of bleomycin.

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Medicine

Preparation of Human Myocardial Tissue for Long-Term Cultivation
Jules Hamers 1,2, Payel Sen 1,2, Daphne Merkus 1,2,3, Thomas Seidel 4,5, Kun Lu 1,6, Andreas Dendorfer 1,2
1Walter-Brendel-Centre of Experimental Medicine, University Hospital, LMU Munich, 2German Center for Cardiovascular Research (DZHK), Partner Site Munich, Munich Heart Alliance (MHA), 3Division of Experimental Cardiology, Department of Cardiology, Erasmus MC, University Medical Center Rotterdam, 4Institute of Cellular and Molecular Physiology, Friedrich-Alexander-Universität Erlangen-Nürnberg, 5Muscle Research Center Erlangen (MURCE), Friedrich-Alexander-Universität Erlangen-Nürnberg, 6Department of Cardiac Surgery, University Hospital, LMU Munich

We present a protocol for ex vivo cultivation of human ventricular myocardial tissue. It allows for detailed analysis of contraction force and kinetics, as well as the application of pre- and afterload to mimic the in vivo physiological environment more closely.

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