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2 ARTICLES PUBLISHED IN JoVE

Bioengineering

Multi-Scale Modification of Metallic Implants With Pore Gradients, Polyelectrolytes and Their Indirect Monitoring In vivo

Nihal E. Vrana¹, Agnes Dupret-Bories^1,2, Christophe Chaubaroux¹, Elisabeth Rieger^1,2, Christian Debry^1,2, Dominique Vautier^1,3, Marie-Helene Metz-Boutigue^1,3, Philippe Lavalle^1,3

¹Biomatériaux et Bioingénieriee, INSERM, ²Service Oto-Rhino-Laryngologie, Hôpitaux Universitaires de Strasbourg, ³Faculté de Chirurgie Dentaire, Université de Strasbourg

In this video, we will demonstrate modification techniques for porous metallic implants to improve their functionality and to control cell migration. Techniques include development of pore gradients to control cell movement in 3D and production of basement membrane mimics to control cell movement in 2-D. Also, a HPLC-based method for monitoring implant integration in-vivo via analysis of blood proteins is described.

Biochemistry

Proteome-wide Quantification of Labeling Homogeneity at the Single Molecule Level

Simon Leclerc^1,2, Youri Arntz², Yuichi Taniguchi^1,3

¹Laboratory for Cell Systems Control, RIKEN Center for Biosystems Dynamics Research, RIKEN, ²Laboratoire de Biomatériaux et Bioimagerie, INSERM, ³PRESTO, Japan Science and Technology Agency

Here, we present a protocol to assess the labeling homogeneity for each protein species in a complex protein sample at the single molecule level.